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CPVO-TP/104/2 Final Date: 21/03/2007 European Union
Community Plant Variety Office
PROTOCOL FOR DISTINCTNESS, UNIFORMITY AND STABILITY TESTS
Cucumis melo L.
UPOV Species Code: CUCUM_MEL Adopted on 21/03/2007
CPVO-TP/104/2 Final Date: 21/03/2007 SUBJECT OF THE PROTOCOL
The protocol describes the technical procedures to be followed in order to meet the Council Regulation (EC) No. 2100/94 on Community Plant Variety Rights. The technical procedures have been agreed by the Administrative Council and are based on general UPOV Document TG/1/3 and UPOV Guideline TG/104/5 dated 05/04/2006 for the conduct of tests for Distinctness, Uniformity and Stability. This protocol applies for all varieties of Cucumis melo L. SUBMISSION OF SEED AND OTHER PLANT MATERIAL
The Community Plant Variety Office (CPVO) is responsible for informing the applicant of • the closing date for the receipt of plant material; • the minimum amount and quality of plant material required; • the Examination Office to which material is to be sent. A sub-sample of the material submitted for test will be held in the variety collection as the definitive sample of the candidate variety. The applicant is responsible for ensuring compliance with any customs and plant health requirements. Final dates for receipt of documentation and material by the Examination Office The final dates for receipt of requests, technical questionnaires and the final date or submission period for plant material will be decided by the CPVO and each Examination Office chosen. The Examination Office is responsible for immediately acknowledging the receipt of requests for testing, and technical questionnaires. Immediately after the closing date for the receipt of plant material the Examination Office should inform the CPVO whether acceptable plant material has been received or not. However if unsatisfactory plant material is submitted the CPVO should be informed as soon as possible. The current quality and quantity requirements as well as the final dates for submission of the plant material are available on the CPVO website (www.cpvo.europa.eu) and are published in the CPVO gazette ‘S2'. CPVO-TP/104/2 Final Date: 21/03/2007 Quality of seeds: . Should not be less than the standards laid down for certified seed in Annex II of EC Council Directive 2002/55/EC. Seed treatment:. The plant material must not have undergone any treatment unless the CPVO and the Examination Office allow or request such treatment. If it has been treated, full details of the treatment must be given. Special requirement: . - Labelling of sample: . - Species - File number of the application allocated by the CPVO - Breeder's reference - Examination office's reference (if known) - Name of applicant - The phrase "On request of the CPVO" CONDUCT OF TESTS
A variety collection will be maintained for the purpose of establishing distinctness of the candidate varieties in test. A variety collection may contain both living material and descriptive information. A variety will be included in a variety collection only if plant material is available to make a technical examination. Pursuant to Article 7 of Council Regulation (EC) No. 2100/94, the basis for a collection should be the following: • varieties listed or protected at the EU level or at least in one of the EEA Member • varieties protected in other UPOV Member States; • any other variety in common knowledge. The composition of the variety collection in each Examination Office depends on the environmental conditions in which the Examination Office is located. Variety collections will be held under conditions which ensure the long term maintenance of each accession. It is the responsibility of Examination Offices to replace reference material which has deteriorated or become depleted. Replacement material can only be introduced if appropriate tests confirm conformity with the existing reference material. If any difficulties arise for the replacement of reference material, Examination Offices must inform the CPVO. If authentic plant material of a variety cannot be supplied to an Examination Office the variety will be removed from the variety collection. CPVO-TP/104/2 Final Date: 21/03/2007 Material to be examined Candidate varieties will be directly compared with other candidates for Community plant variety rights tested at the same Examination Office, and with appropriate varieties in the variety collection. When necessary an Examination Office may also include other candidates and varieties. Examination Offices should therefore make efforts to co-ordinate the work with other Offices involved in DUS testing of melon. There should be at least an exchange of technical questionnaires for each candidate variety, and during the test period, Examination Offices should notify each other and the CPVO of candidate varieties which are likely to present problems in establishing distinctness. In order to solve particular problems Examination Offices may exchange plant material. Characteristics to be used The characteristics to be used in DUS tests and preparation of descriptions shall be those referred to in the Annex I. All the characteristics shall be used, providing that observation of a characteristic is not rendered impossible by the expression of any other characteristic, or the expression of a characteristic is prevented by the environmental conditions under which the test is conducted. In the latter case, the CPVO should be informed. In addition the existence of some other regulation e.g. plant health,, may make the observation of the characteristic impossible. The Administrative Council empowers the President, in accordance with Article 23 of Commission Regulation (EC) No. 1239/95, to insert additional characteristics and their expression in respect of a variety. Grouping of varieties The varieties and candidates to be compared will be divided into groups to facilitate the assessment of distinctness. Characteristics which are suitable for grouping purposes are those which are known from experience not to vary, or to vary only slightly, within a variety and which in their various states of expression are fairly evenly distributed throughout the collection. In the case of continuous grouping characteristics overlapping states of expression between adjacent groups is required to reduce the risks of incorrect allocation of candidates to groups. The characteristics used for grouping could be the following: a) Inflorescence: sex expression (at full flowering) (characteristic 12) b) Fruit: length (characteristic 24) c) Fruit: shape in longitudinal section (characteristic 28) d) Fruit: ground colour of skin (characteristic 29) e) Fruit: density of patches (characteristic 36) f) Fruit: grooves (characteristic 43) g) Fruit: cork formation (characteristic 48) CPVO-TP/104/2 Final Date: 21/03/2007 h) Fruit: pattern of cork formation (characteristic 50) i) Fruit: main colour of flesh (characteristic 54) j) Seed: length (characteristic 59) k) Seed: colour (characteristic 62) l) Resistance to race 0 of Fusarium oxysporum f. sp melonis (characteristic 68.1) m) Resistance to race 1 of Fusarium oxysporum f. sp melonis (characteristic 68.2) n) Resistance to race 2 of Fusarium oxysporum f. sp melonis (characteristic 68.3) Trial designs and growing conditions The minimum duration of tests will normally be two independent growing cycles. Tests will be carried out under conditions ensuring normal growth. The size of the plots will be such that plants or parts of plants may be removed for measuring and counting without prejudice to the observations which must be made up to the end of the growing period. The test design is as follows Each test should include 20 plants divided between two or more replicates. All observations determined by measurement or counting should be made on 20 plants or parts of 20 plants. In accordance with Article 83(3) of Council Regulation (EC) No. 2100/94 an applicant may claim either in the Technical Questionnaire or during the test that a candidate variety has a characteristic which would be helpful in establishing distinctness. If such a claim is made and is supported by reliable technical data, a special test may be undertaken providing that a technically acceptable test procedure can be devised. Special tests will be undertaken, with the agreement of the President of CPVO, where distinctness is unlikely to be shown using the characteristics listed in the protocol. Standards for decisions
a) Distinctness
A candidate variety will be considered to be distinct if it meets the requirements of Article
7 of Council Regulation (EC) No. 2100/94.
CPVO-TP/104/2 Final Date: 21/03/2007
b) Uniformity
Self-pollinated varieties, hybrid varieties and vegetatively propagated varieties will be
considered to be sufficiently uniform if the number of off-types does not exceed the
number of plants as indicated in the table below. A population standard of 1% and an
acceptance probability of 95% should be applied.
Table of maximum numbers of off-types allowed for uniformity standards.
Number of plants off-types allowed
For the assessment of uniformity of open-pollinated varieties, relative uniformity standards
should be used.
c) Stability
A candidate will be considered to be sufficiently stable when there is no evidence to
indicate that it lacks uniformity.
REPORTING OF RESULTS
After each recording season the results will be summarised and reported to the CPVO in the form of a UPOV model interim report in which any problems will be indicated under the headings distinctness, uniformity and stability. Candidates may meet the DUS standards after two growing periods but in some cases three growing periods may be required. When tests are completed the results will be sent by the Examination Office to the CPVO in the form of a UPOV model final report. If it is considered that the candidate complies with the DUS standards, the final report will be accompanied by a variety description in the format recommended by UPOV. If not the reasons for failure and a summary of the test results will be included with the final report. The CPVO must receive interim reports from the Examination Office and final reports by the date agreed between the CPVO and the Examination Office. Interim reports and final examination reports shall be signed by the responsible member of the staff of the Examination Office and shall expressly acknowledge the exclusive rights of disposal of CPVO. CPVO-TP/104/2 Final Date: 21/03/2007 LIAISON WITH THE APPLICANT
If problems arise during the course of the test the CPVO should be informed immediately so that the information can be passed on to the applicant. Subject to prior agreement, the applicant may be directly informed at the same time as the CPVO particularly if a visit to the trial is advisable. The interim report as well as the final report shall be sent by the Examination Office to the CPVO. CPVO-TP/104/2 Final Date: 21/03/2007 ANNEXES TO FOLLOW
Table of characteristics . 10 Explanations and methods . 25 Legend: Note: For the CPVO numbered characteristics, all characteristics in the table are compulsory; notwithstanding, in the case of disease resistance characteristics, only those resistances marked with an asterisk (*) in the CPVO column are compulsory. The asterisks in the UPOV numbered characteristics are there for information purposes and denote those characteristics which should always be observed when a UPOV guideline is utilised. In general for the assessment of resistance characteristics, the facilities of other Examination Offices or specialised institutions might be used, subject to previous arrangements. Some characteristics may be discarded: if there are already phytosanitary restrictions. (+) See explanations on the Table of characteristics (a) – (e) See explanations on the table of characteristics Types of expression of characteristics: QL – Qualitative characteristic QN – Quantitative characteristic PQ – Pseudo-qualitative characteristic Type of observation of characteristics: MG – Single measurement of a group of plants or parts of plants MS – Measurement of a number of individual plants or parts of plants VG – Visual assessment by a single observation of a group of plants or parts of plants VS – Visual assessment by observation of individual plants or parts of plants When a method of observation is attributed to a certain characteristic, the first differentiation is made depending if the action taken is a visual observation (V) or a measurement (M). The second differentiation deals with the number of observations the expert attributes to each variety, thus the attribution of either G or S. If a single observation of a group consisting of an undefined number of individual plants is appropriate to assess the expression of a variety, we talk about a visual observation or a measurement made on a group of plants, thus we attribute the letter G (either VG or MG). If the expert makes more than one observation on that group of plants, the decisive part is that we have at the end only one data entry per variety which means that we have to deal with G (e.g. measurement of plant length on a plot – MG, visual observation of green colour of leaves on a plot – VG). CPVO-TP/104/2 Final Date: 21/03/2007 If it is necessary to observe a number of individual plants to assess the expression of a variety, we should attribute the letter S (thus either VS or MS). Single plant data entries are kept per variety for further calculations like the variety mean (e.g. measurement of length of ears – MS, visual observation of growth habit of single plants in grasses – VS). The number of individual plants to be observed in such cases is stated in section III.5. Literature . 40 Technical Questionnaire CPVO-TP/104/2 Final Date: 21/03/2007 TABLE OF CHARACTERISTICS TO BE USED IN DUS TESTS AND
PREPARATION OF DESCRIPTIONS
Examples
Seedling: length of hypocotyl
(a) (a) VG very short
Seedling:
cotyledon
(a) (a) VG very small
Seedling: intensity of green colour of
cotyledon
(a) (a) VG light Bimbo,
Candy, Piel de Sapo (b) (b) VG small Geaprince,
intensity of green colour
(b) (b) VG light Fimel,
CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Leaf blade: development of lobes
(+) (+) VG weak Boule
Leaf blade: length of terminal lobe
(+) (+) VG short Perlita
Leaf blade: dentation of margin
(b) (b) VG weak Clipper,
De Cavaillon espagnol à chair rose, Piel de Sapo Boule d'or, Portoluz blistering
(b) (b) VG weak Galia
Petiole: attitude
(b) (b) VG erect Alfredo
semi-erect Peko 3 horizontal Creso 5 11. 11. QN
Petiole:
Inflorescence: sex expression (at full
flowering)
VG monoecious Alpha,
G andromonoecious Piel
CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Young fruit: hue of green colour of skin
(+) (+) VG whitish green
Young fruit: intensity of green colour of
(c) (*) VG very light
(c) light Fimel
Young fruit: density of dots
(c) (c) VG
absent or very sparse Young fruit: size of dots
(c) (c) VG
Young fruit: contrast of dot
colour/ground colour
(c) (c) VG
CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Young fruit: conspicuousness of groove
colouring
(c) (c) VG
absent or very weak Young fruit: intensity of groove colouring
(c) (c) VG
Young fruit: length of peduncle
Young fruit: thickness of peduncle
1 cm from fruit
medium Geaprince, thick Charentais, Young fruit: extension of darker area
around peduncle
(c) (c) VG
absent or very small CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Fruit: change of skin colour from young
fruit to maturity
(+) (+) VG
early in fruit development Alpha, Charentais, Clipper late in fruit development Amarillo Oro, Galia very late in fruit development or no Futuro, Piel de Sapo 24. 24. QN
Doublon, Golden Crispy (d) short Topper,
long Categoría, G very long
Katsura Giant, Valdivia 25. 25. QN
Fruit: diameter
Banana, Golden Crispy (d) narrow Alpha,
medium Categoría, 26. 26. QN
(d) very small to small
large Categoría, large to very large CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Fruit: position of maximum diameter
(+) (+) VG toward stem end
Piolín , Sapo de Oro (*) at middle
Piel de Sapo, Védrantais toward blossom end Cganchi, Edén, Katsura Giant Fruit: shape in longitudinal section
(+) (+) VG ovate
De Cavaillon, Piolín (*) medium elliptic
Jívaro, Noir de Carmes G elongated Alficoz,
Fruit: ground colour of skin
(+) (+) VG white
Albino, Honey Dew (*) yellow
Amarillo-Canario, Edén, Galia, Passport, Solarking Gohyang, Piel de Sapo Geaprince, Geamar, Romeo, Sirio, Supporter, Védrantais Fruit: intensity of ground colour of skin
(d) (d) VG light 3
CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Fruit: hue of ground colour of skin
(+) (+) VG absent or very weak
Amarillo-Canario, Albino, Piel de Sapo, Sirio yellowish Geaprince, Geamar, Honey Dew, Solarking Fruit: density of dots
(d) (d) VG absent or very sparse
Petit Gris de Rennes Fruit: size of dots
(d) (d) VG small Doral
Fruit: colour of dots
(d) (d) VG white Edén
Fruit: intensity of colour of dots
(d) (d) VG light Kinka,
CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Fruit: density of patches
VG absent or very sparse
G very dense
Fruit: size of patches
(d) (d) VG small Baltasar
VG absent Piel
Fruit: strength of attachment of peduncle
at maturity
VG very weak
Doral, Védrantais Fruit: shape of base
(+) (+) VG pointed Edén 1
(*) rounded Arava 2
truncate Zatta 3 Fruit: shape of apex
(+) (+) VG pointed Canador,
(*) rounded
Alpha, Honey Dew CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Fruit: size of pistil scar
VG small Alpha,
Charentais, Eros, Verdol 43. 43. QL
VG absent or very weakly expressed
Piel de Sapo, Arava weakly expressed G strongly expressed
Védrantais, Galia Fruit: width of grooves
(d) (d) VG narrow Auraprince
Fruit: depth of grooves
(d) (d) VG very shallow
Noir des Carmes, Sucrin de Tours Fruit: colour of grooves
(d) (d) VG white Geumssaraki
green Charentais Fruit: creasing of surface
(+) (+) VG absent or very weak
(*) weak Melchor,
Balbey, Kirkagac CPVO-TP/104/2 Final Date: 21/03/2007 Examples
48. 48. QL
formation
(d) (*) VG absent Alpha
G (d) present Dalton
Fruit: thickness of cork layer
VG very thin
Honey Rock, Perlita Fruit: pattern of cork formation
VG dots only
Hermes, Védrantais linear and netted G netted only
Fruit: density of pattern of cork
formation
(d) (d) VG very sparse
Alpha, Amarillo Oro sparse Védrantais Honey Rock, Perlita Fruit: rate of change of skin colour from
maturity to over maturity
(+) (+) VG absent or very slow
Clipper, Doral, Galia, Honey dew, Corin, Marina, Nembo CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Fruit: width of flesh in longitudinal
section (at position of maximum fruit
diameter)
(+) (+) VG thin Gama
Fruit: main colour of flesh
VG white Piel
orange Védrantais G reddish orange
Only varieties with main colour of flesh:
orange: Fruit: intensity of orange colour
of flesh
(d) (d) VG light Fantasy,
Only varieties with main colour of flesh:
white; greenish white; green; yellowish
white: Fruit: secondary salmon colouring
of flesh
(d) (d) VG absent or very weak
weak Auraprince, CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Only varieties with change of skin colour
from maturity to over maturity: Fruit at
over maturity: hue of colour of skin
creamish Figaro, Only varieties with change of skin colour
from maturity to over maturity and with
yellow or orangish yellow colour of skin:
Fruit at over maturity: intensity of yellow
colour of skin
59. 60. QN
Geumssaraki, Golden Crispi Elario, Katsura Giant G very long
60. 61. QN
61. 62. QL
(+) (+) VG not pine-nut shape
CPVO-TP/104/2 Final Date: 21/03/2007 Examples
62. 63. QL
(e) (*) VG whitish
Amarillo Oro s.b. G (e) cream yellow
Galia, Piel de Sapo Only varieties with cream yellow seed
colour: Seed: intensity of colour
Time of male flowering
medium Categoría Time of female flowering
Braco, Categoría, Vital Time of ripening
medium Védrantais Pinonet, Piel de Sapo, Rochet Clipper, Supporter, Tendral 67. 68. QN
(+) (+) MG very short
(*) short Galia
CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Resistance to Fusarium oxysporum f. sp.
68.1 69.1 Race
(*) absent Jaune
G present Jador,
68.2 69.2
(*) absent
Jaune Canari 2, Védrantais G present Jador,
68.3 69.3
(*) absent
Jaune Canari 2, Joker G present Jador,
68.4 69.4 Race
Jaune Canari 2 Joker, Védrantais Resistance to Sphaerotheca fuliginea
(Podosphaera xanthii) (Powdery mildew)
69.1 70.1 Race
susceptible Alpha, Boneto, Delta, Jerac intermediate resistant highly resistant Cézanne, Anasta, Théo 69.2 70.2 Race
susceptible Boneto, intermediate resistant Flores, Enzo, Escrito highly resistant Anasta, Cézanne, Théo 69.3 70.3 Race
susceptible Védrantais intermediate resistant highly resistant Gaetano, Lucas, Théo CPVO-TP/104/2 Final Date: 21/03/2007 Examples
Resistance to Erysiphe cichoracearum
(Golovinomyces cichoracearum)
Race 1 (Powdery mildew)
susceptible Bastion, intermediate resistant highly resistant Cézanne, Heliobel, Théo Resistance to colonization by Aphis
gossypii
absent Charentais AR, Margot, Top Mark Resistance to Zucchini Yellow Mosaic
Virus (ZYMV)
Race F
Alpha, Boule d'Or, Cantor, Resistance to Papaya Ring Spot Virus
73.1 74.1 Race
absent Védrantais 73.2 74.2 Race
absent Védrantais, Resistance to Muskmelon Necrotic Spot
Virus (MNSV)
Race E8
absent Védrantais Resistance to Cucumber Mosaic Virus
absent Cézanne, present Lunaduke CPVO-TP/104/2 Final Date: 21/03/2007 EXPLANATIONS AND METHODS
Explanations covering several characteristics
(a) Seedling: All observations on the seedling should be made just before the development of the first true leaf. blade: Unless otherwise indicated, all observations on the leaf blade should be made on fully developed but not old leaves, preferably between the 5th and 8th node when the plant has at least 11 nodes. fruit: All observations on the young fruit should be made on green, unripe fruits, before the colour change. For most varieties this means when the fruit is half the final size. To facilitate the observation, it is recommended to harvest one young fruit per plant, if the number of fruits per plant makes that possible. (d) Fruit: Observations which should be made on ripened fruit. The colour must not start to change to the over maturity colour. When appropriate, for the flesh characteristics it is recommended to wait at least one week after the harvest before opening the fruits. (e) Seed: All observations on the seed should be made on fully developed and dry seeds, after washing and drying in the shade. Ad. 6: Leaf blade: development of lobes

CPVO-TP/104/2 Final Date: 21/03/2007 Ad. 7: Leaf blade: length of terminal lobe Ad. 13: Young fruit: hue of green colour of skin The basic colour of the young fruit is green. There are two true hue levels "yellowish" and "green" depending on the proportion between red and blue components in the colour, and two other hue levels "greyish" that is rather a low saturation of the green colour and "whitish" that results from a very light intensity of the green colour. Ad. 23: Fruit: change of skin colour from young fruit to maturity Ad. 52: Fruit: Rate of change of skin colour from maturity to over maturity The melon fruit may have up to three different skin colours in the course of its development. The speed of evolution of the colour depends on the type of variety, but within a type different speeds can also be observed. Please note that in cases where the colour change is closely linked to maturity, the observation should be clear: either on the colour change related to maturity (characteristic 23) or within mature fruits from mature to over mature (characteristic 52). The changing of fruit skin colour can be described by using the following characteristics: 1. Stage 1: colour of the young fruit (green colour) 2. Change from Stage 1 to Stage 2 (Characteristics 23) 3. Stage 2: colour at maturity 4. Change from Stage 2 to Stage 3 (Characteristic 52) 5. Stage 3: colour at over maturity.

CPVO-TP/104/2 Final Date: 21/03/2007 Some examples are given in the following table: Variety Stage colour of the Stage 1 to Amarillo Oro green Doral green late Charentais green Alpha green early grey medium yellow Clipper green early Corin green early Nembo green early Albino green late white absent white Marina green no-change green medium yellow Ad. 27: Fruit: position of maximum diameter toward blossom end

CPVO-TP/104/2 Final Date: 21/03/2007 Ad. 28: Fruit: shape in longitudinal section
Ad. 29: Fruit: ground colour of skin
Ad. 31: Fruit: hue of ground colour of skin
For example:
All the Galia type would be considered as yellow colour. Hues ochre, orange, pure yellow or
greenish can be considered in the group, but in a separate characteristic (31).
All the Charentais type would be considered as grey. Greenish, whitish, or yellowish hues
(characteristic 31) can be used for distinctness, but are not recommended for grouping.

Ochre is pale brownish yellow.
CPVO-TP/104/2 Final Date: 21/03/2007 The colours given below indicate the ground colour of skin of the variety in question. Hue of ground colour (characteristic 31) (characteristic 29) Amarillo-Canario Solarking yellow
Ad. 40: Fruit: shape of base
CPVO-TP/104/2 Final Date: 21/03/2007 Ad. 41: Fruit shape of apex Ad. 47: Fruit: creasing of surface Ad. 52: Fruit: Rate of change of skin colour from maturity to over maturity See Ad. 23, Ad. 52 Ad. 53: Fruit: width of flesh in longitudinal section (at position of maximum fruit diameter) CPVO-TP/104/2 Final Date: 21/03/2007 Ad. 61: Seed: shape (a) cross section (b) longitudinal section not pine-nut shape Pine-nut shape seed (Piñonet) is controlled by a recessive characteristic with simple genetic regulation. Seed with pine-nut shape resembles the shape of a pine nut and has the following features: the hilum end is slightly more pointed, with very small wings; the apical end has a tendency to be more rounded; in cross section the seed has a tendency to be more symmetrically elliptical; the surface is not covered with arista. Ad. 67: Shelf life of fruit Shelf life is the time that the fruit remains firm in storage. Five fruits per plot are stored in boxes in single layers. The boxes can be stored one on top on another if air can circulate between them. The storage area does not need to be climatically controlled, but must have naturally good conditions for storing fruits. Observations are made at regular intervals of 3 to 4 days, noting the firmness of fruits, taking care not to damage them, and removing those which are damaged or rotten. The observation is to determine when the fruits become soft, i.e. when the firmness of the fruit becomes equal or lower than Note 3 "soft" in characteristic 57. Ads. 68.1 - 68.3: Resistance to Fusarium oxysporum f. sp. melonis, races 0, 1 and 2 Maintenance of races on agar medium at 22 to 25°C Special conditions: transplantation of races each month CPVO-TP/104/2 Final Date: 21/03/2007 Execution of test Growth stage of plants: cotyledons expanded 24°C during day, 18°C during night 10-12 hours per day Petri dishes in climatic chambers Method of inoculation: soaking of the root system in a suspension of liquid medium of fungus Duration of test - from sowing to inoculation: - from inoculation to reading: 20 days, death of susceptible plants Number of plants tested: plants raised and transplanted in sterilized sand, irrigation with nutrient solution Ad. 68.4: Resistance to Fusarium oxysporum f. sp. melonis, race 1-2 Maintenance of races on agar medium at 22 to 25°C Special conditions: transplantation of races each month Execution of test Growth stage of plants: cotyledons expanded 24°C during day, 18°C during night 12 hours per day dishes in climatic chambers Method of inoculation: absorption of 700 ml of a very diluted (30 to 50 times) fungus culture Duration of test - from sowing to inoculation: - from inoculation to reading: 3 weeks, until the death of the susceptible control Number of plants tested: a moderately aggressive type of race 1-2 should be used as this is likely to show the difference between the presence and absence of resistance most clearly. CPVO-TP/104/2 Final Date: 21/03/2007 Ads. 69.1 to 69.3: Resistance to Sphaerotheca fuliginea (Podosphaera xanthii), races 1, 2 and 5 Ad. 70: Resistance to Erysiphe cichoracearum (Golovinomyces cichoracearum), race 1 1. Inoculum Production of cotyledons Cotyledons to be inoculated and tested: sow the seed in disinfected peat inside a closed mini glasshouse. When the cotyledons have expanded, remove them from the plant. Desinfect the cotyledons by soaking them for 3 minutes in a mercuric chloride solution (0.05%). Rinse them twice with sterilized water. Dry the cotyledons with sterile paper towel, then place them in Petri dishes with the following medium: sucrose Propagation of the strains Scatter conidia on the cotyledons and blow them. Incubate the inoculated cotyledons in Petri dishes at 23oC during 14 hours in the light and at 18oC during 10 hours in the dark. 9 to 11 days after the inoculation, the cotyledons will be covered with spores and can be used as an inoculum. Maintenance of races Type of medium: on inoculated cotyledons Special conditions: 17oC, under very low light intensity. Maximum storage time is 1 to 1.5 months, after the inoculation. Execution of Test Inoculation on leaf disks (to be used as routine method) Leaf disks, 2 cm in diameter, are taken from young plants and placed in polystyrene boxes (180 x 125 mm, 54 leaf disks per box) on a medium (mannitol 40g/l, benzamidazole 30 mg/l, agar 4 g/l). The leaf disks are inoculated by placing the boxes at the base of an inoculation tower (height: 1.00 m, diameter 0.25 m). A cotyledon, already covered with inoculum, is placed on the top of the tower and blown with a Pasteur pipette to detach spores. Wait 1 to 2 minutes so that the conidia fall down through the tower onto the leaf discs. The leaf disks are kept for 24 hours in the dark by covering the boxes with a black polyethylene sheet. The boxes are then placed in a climatised chamber (20oC in the light for 14 hours; 24oC in the dark, for 10 hours per day). CPVO-TP/104/2 Final Date: 21/03/2007 Duration of test/Number of plants - from inoculation to reading: - number of plants tested: Highly resistant varieties (Note 3) 0 no development of the fungi isolated colonies (less than 10% of the disk surface) Intermediate resistant varieties (especially for Erysiphe cichoracearum (Golovinomyces cichoracearum)) (Note 2) isolated colonies (more than 10 % of the disk surface) all the disk surface is covered with weak sporulation Susceptible varieties (Note 1) 4 sporulation on all the disk surface Inoculation on young plants (to be used as a complementary method to the disk method, if necessary) Take spores from a cotyledon already covered with conidia and deposit them on a leaf taken from a young plant. You can also proceed by blowing the spores from a cotyledon by the method mentioned above. Scoring Highly resistant varieties (Note 3) 0 no development of the fungi isolated colonies (less than 10% of the leaves) Intermediatel resistant varieties (especially for Erysiphe cichoracearum (Golovinomyces cichoracearum)) (Note 2) isolated colonies (more than 10% of the leaves) Susceptible varieties (Note 1) 7 medium CPVO-TP/104/2 Final Date: 21/03/2007 Sphaerotheca fuliginea
(Podosphaera xanthii)
S: susceptible (high sporulation) R: resistant (low sporulation) Ad. 72: Resistance to colonization by Aphis gossypii Maintenance of strain Maintenance and multiplication: on susceptible variety (Védrantais) Special conditions: low aphid density to avoid having too many winged types. "Synchronous"-type breeding in order to have only aphids of the same age and, therefore, at the same growing stage on a plant. Conduct of the test 1st leaf measuring 2-3 cm Temperature: 21oC Light: 16 hours per day plants sown in sand, pricked out at cotyledon stage in compost-filled pots Manner of inoculation: deposit of ten adult wingless aphids per plant Duration of test: - from sowing to inoculation: - from inoculation to reading: Number of plants tested: - Resistance present = less than 7 adult aphids per plant; eggs rare. - Resistance absent = 9 or 10 adult aphids per plant; eggs frequent. - Record number of aphids per plant, 24 hours after inoculation. CPVO-TP/104/2 Final Date: 21/03/2007 Ad. 73: Resistance to Zucchini Yellow Mosaic Virus (ZYMV), race F A. INOCULUM Maintenance of strain Maintenance: 5oC and kept dry using anhydrous calcium chloride Special conditions: pre-multiplication of the virus on non-wilting variety (Védrantais) prior to testing INOCULATION AND INCUBATION Conduct of the test 1st emergent leaf Temperature: 25oC during day, 18oC during night Light: 12 hours per day Manner of inoculation: mechanical inoculation by rubbing of cotyledons with inoculum Duration of test: - from sowing to inoculation: - from inoculation to reading: Number of plants tested: Reading difficulty: - heterozygotes (Fn/Fn+) wither and die more slowly than homozygotes (Fn/Fn) - use the F pathotype of ZYMV Example varieties: Védrantais (Fn+/Fn+): mosaic (resistance present) Cantor (Fn/Fn+): slower necrosis with wilting (resistance absent) Doublon (Fn/Fn): necrosis with wilting (resistance absent) Ad. 74: Resistance to Papaya Ring Spot Virus (PRSV), race GVA and race E2 A. INOCULUM Maintenance of strain 5oC and kept dry using anhydrous calcium chloride Special conditions: pre-multiplication of the virus on susceptible variety (Védrantais) prior to testing CPVO-TP/104/2 Final Date: 21/03/2007 INOCULATION AND INCUBATION Conduct of the test 1st emergent leaf Temperature: 25oC during day, 18oC during night Light: 12 hours per day Manner of inoculation: mechanical inoculation by rubbing cotyledons with inoculum Duration of test: - from sowing to inoculation: - from inoculation to reading: Number of plants tested: Identification of two strains of the PRSV virus and of the two alleles concerned: Genotypes/Strains Mosaic (vein-clearing) Mosaic (vein-clearing) = resistance absent = resistance absent - No systemic symptoms - Local necrotic lesions on Necrosis of plant instead cotyledons (irregular) of local lesions: resistance = resistance present - No systemic symptoms - No systemic symptoms lesions on cotyledons lesions on cotyledons = resistance present = resistance present Ad. 75: Resistance to Muskmelon Necrosis Spot Virus (MNSV), race E8 A. INOCULUM Maintenance of strain Maintenance: 5oC and kept dry using anhydrous calcium chloride Special conditions: pre-multiplication on susceptible variety (Védrantais) prior to test CPVO-TP/104/2 Final Date: 21/03/2007 INOCULATION AND INCUBATION Conduct of the test 1st emergent leaf Temperature: 25oC during day, 18oC during night Light: 12 hours per day Manner of inoculation: mechanical inoculation by rubbing of cotyledons with inoculum Duration of test: - from sowing to inoculation: 15 days - from inoculation to reading: 8 days Number of plants tested: Susceptible plants: necrotic lesions on the inoculated organs (cotyledons) Resistant plants: Ad. 76: Resistance to Cucumber Mosaic Virus (CMV) A. INOCULUM 1. Crushed Sodium hydrogen phosphate (Na2HPO4, 12 H2O) (0,03M): Diethyldithiocarbamate of sodium (= DIECA): Distilled water: The sodium hydrogen phosphate solution can be stored in a refrigerator. Once the DIECA is added, the solution should be used within the next two hours. 2. Crushing the leaves The source of the inoculum comes from crushing either the fresh leaves, or leaves desiccated in anhydrous calcium chloride (Ca Cl2), in a cold mortar. Crush 1 gram of leaves with 4 ml of phosphate disodic solution at 5°C. Add active carbon (0,5 g) and carborendum (0,4 g) for each 1 gram of leaves. After crushing, put the mortar on a bed of ice. Before using leaves dried with CaCl2 to inoculate a plant test, do a multiplication of the inoculum on some 10 susceptible plants which would be used as inoculum. CPVO-TP/104/2 Final Date: 21/03/2007 CMV can be stored for several years by desiccation with anhydrous CaCl2. Leaves showing mosaic symptoms should be chopped finely with a razor blade and placed in cups. Put a layer of anhydrous calcium chloride (0,5 cm) in a plastic box and cover it with filter paper. Place the cups on this layer. Close the box well with adhesive tape, and then place it in a tightly closed plastic bag. Store it in a refrigerator at 5°C. B. INOCULATION AND INCUBATION Cotyledons or young leaves should be inoculated by rubbing them with a latex-protected finger. After a few minutes, rinse the cotyledons with running water. Place the plants for incubation in a growth chamber (generally at 18°C at night and 25°C in the day, with 12 to 14 hours of daylight). C. SYMPTOMS The "common" strains of CMV bring out mosaic symptoms on susceptible plants one week after inoculation. Resistant plants show no symptoms. Remarks: When light intensity and daylight are not sufficient (winter period), resistant plants (in particular PI 161375) may present chlorotic lesions on the first leaf. Strains: Use "common" strains (as T1, P9) rather than "song" strains (14, T2). CMV common strains (T1, P9) CMV song strains (14, T2) Susceptible Védrantais mosaic, chlorotic lesions P9 brings out "aucuba" mosaic on susceptible varieties P9 is less aggressive than T1 It is preferable to use Virgos rather than PI 161375 (lower germination, weaker growth). Observations, notes: The genetic resistance is polygenic. Use a notation with classes. It is preferable to use the two strains P9 and T1 to have a better evaluation of the resistance. High resistance confers resistance on all common strains. Some genotypes may present a resistance to P9 (no symptoms), and a slight susceptibility to T1 (slight mosaic). CPVO-TP/104/2 Final Date: 21/03/2007 LITERATURE
Besombes, D.; Giovinazzo, N.; Olivier, C.; Dogimont, C.; itrat, M., 1999: Description and inheritance of an albino mutant in melon, Cucurbit Genetics Cooperative Report (USA), no. 22; 14-15 El Tahir, I.M.; Pitrat, M., 1999: Tibish, a melon type from Sudan, Cucurbit Genetics Cooperative Report (USA), no. 22; 21-23. Guis, M.; Roustan, J.P.; Dogimont, C.; Pitrat, M.; Pech, J.C., 1998: Melon biotechnology, Biotechnology and Genetic Engineering Reviews (GBR), vol. 15; 289-311. Guis, M.; Botondi, R.; Ayub, R.; Ben Amor, M.; Guillen, P.; Latché, A.; Bouzayen, M.; Pech, J.C.; Dogimont, C.; Pitrat, M.; Lelièvre, J.M.; Albagnac, G., 1996: Physiological and biochemical evaluation of transgenic cantaloupe charentais melons with reduced levels of ACC oxidase, EUCARPIA; European Association for Research on Plant Breeding; Paris (FRA); Cucurbits towards 2000, 5. Eucarpia Meeting on Cucurbit Genetics and Breeding; Malaga (ESP); 1996/05/28-30, 194-199, EUCARPIA; Paris (FRA). Le Couviour, M.; Pitrat, M.; Olivier, C.; Ricard, M., 1995: Cochleare folium, a mutant with spoon-shaped leaf in melon, Cucurbit Genetics Cooperative (USA), no. 18; 37. Périn, C.; Gomez-Jimenez, M.C.; Hagen, L.; Dogimont, C.; Pech, J.C.; Latché, A.; Lelièvre, J.M.; Pitrat, M., 2002: Genetic control of fruit quality and maturation traits in melon, ISHS; International Society for Horticultural Science; Cucurbit Working Group; (NLD); Cucurbits. Abstracts 2. International Symposium; Tsukuba (JPN); 2001/09/28; 2001/10/01, 1p. Perin, C.; Dogimont, C.; Giovinazzo, N.; Besombes, D.; Guitton, L.; Hagen, L.; Pitrat, M., 1999: Genetic control and linkages of some fruit characters in melon, Cucurbit Genetics Cooperative Report (USA), no. 22; 16-18. Périn, C.; Gomez, M.C.; Lelièvre, J.M.; Valentin, M.; Vaissière, B.; Gary, C.; Dogimont, C.; Causse, M.; Pech, J.C.; Pitrat, M., 1999: Contrôle génétique et éco-physiologique de l'élaboration de la qualité chez le melon Cucumis melo L., Abagnac, G.; Colonna, P.; Doussinault, G.; Habib, R.; INRA; Institut National de la Recherche Agronomique; Paris (FRA); AIP-AGRAF pour l'élaboration de la composition et de l'aptitude à l'utilisation des grains et des fruits 1996-1999, 97-116. Pitrat, M., 2002: 2002 gene list for melon, Cucurbit Genetics Cooperative Report (USA), no. 25; 76-93. Pitrat, M.; Hanelt, P.; Hammer, K., 2000: Some comments on intraspecific classification of cultivars of melon, Katzir, N. (ed.); Paris, H.S. (ed.); ISHS; International Society for Horticultural Science; Working Group on Cucurbitaceae; Wageningen (NLD); Cucurbitaceae 2000. Proceedings; Acta Horticulturae (NLD), 7. EUCARPIA Meeting on Cucurbit genetics and breeding; Ma'ale Ha Hamisha (ISR); 2000/03/19-23, no. 510; 29-36, ISHS; Wageningen (NLD). Pitrat, M., 1998: 1998 gene list for melon, Cucurbit Genetics Cooperative Report (USA), no. 21; 69-81. CPVO-TP/104/2 Final Date: 21/03/2007 Pitrat, M.; Dogimont, C.; Périn, C.; Hagen, L.; Burget, E.; Gomez Jimenez, M.C.; Mohamed, E.T.I.; Yousif, M.T.; Riffaud, C.; Rode, J.C., 2001: Recherches sur le melon, INRA; Centre d'Avignon; Unité de Génétique et d'Amélioration des Fruits et Légumes; Montfavet (FRA); Rapport d'activités 1997-2000, 39-45 Pitrat, M., 1998: Deux nouvelles techniques utilisées pour l'amélioration du melon, PHM Revue Horticole (FRA), no. 11; 6-7. Pitrat, M.; Dogimont, C.; Baudracco-Arnas, S.; Cabasson, C.; Rode, J.C.; Carré, M., 1995: Recherches sur le melon, INRA; Centre de Recherche d'Avignon; Station d'Amélioration des Plantes Maraîchères; Montfavet (FRA); Rapport d'activités 1993-1994, 31-40, INRA Editions; Paris (FRA). Pitrat, M.; Olivier, C.; Ricard, M., 1995: A virescent mutant in melon, Cucurbit Genetics Cooperative (USA), no. 18; 37. Pitrat, M., 1995: Interaction between monoecy and male sterility in melon, Cucurbit Genetics Cooperative (USA), no. 18; 38-39. Pitrat, M.; Risser, G., 1992: Le melon, Gallais, A. (ed.); Bannerot, H. (ed.); Amélioration des espèces végétales cultivées. Objectifs et critères de sélection, 448-459, INRA; Paris (FRA). Pitrat, M.; Risser, G.; Maestro, C.; Epinat, C., 1991: Recherches sur le melon, Rapport d'activité 1991, no. 89-90; 27-34. Pitrat, M.;Risser, G.; Ferriere, C.; Olivier, C.; Ricard, M., 1991: Two virescent mutants in melon (Cucumis melo L.), Cucurbit Genetics Cooperative (USA), no. 14; 45. Risser, G.; Rode, J.C., 1988: Natural parthenocarpy observed on melon cv. "Dvash Ha Ogen", Risser, G. (Ed.); Pitrat, M. (Ed.); EUCARPIA; European Association for Research on Plant Breeding; Montfavet (FRA); Cucurbitaceae 88. Proceedings of the EUCARPIA meeting on Cucurbit Genetics and Breeding, Cucurbitaceae 88; Montfavet (FRA); 1988/05/31-1988/06/01-02, 113-114, INRA; Paris (FRA). Risser, G., 1986: Maternal effect on growth of melon seedlings, Cucurbit Genetics Cooperative (USA), no. 9; 2 p. DISEASE RESISTANCE Bardin, M.; Perchepied, L.; Dogimont, C.; Nicot, P.; Pitrat, M., 2002: Analyse génétique de la résistance à l'oïdium chez le génotype de melon PI 124112, CNRS; CAES; Aussois (FRA); Journées Jean Chevaugeon, 4. Rencontres de Phytopathologie/Mycologie; Aussois (FRA); 2002/03/13-17, 1 p. Bardin, M.; Pitrat, M.; Nicot, P.C., 2002: Oïdium du melon. Biologie et méthodes de lutte, Le Maraîcher (FRA); suppl. de PHM Revue Horticole, no. 436; 16-19. CPVO-TP/104/2 Final Date: 21/03/2007 Bardin, M.; Dogimont, C.; Pitrat, M.; Nicot, P.C., 1999: Virulence of Sphaerotheca fuliginea and Erysiphe cichoracearum on melon and genetic analysis of resistance of melon genotypes ‘PI 124112' and ‘PI 414723'. (poster), Bélanger, R.R.; Bushnel, W.R.; Carver, W.R.; Dik, A.J.; Kunoh, H.; Nicot, P.; Schmitt, A.; Powdery mildew. Programme and abstracts, 1. Conférence; Avignon (FRA); 1999/08/29; 1999/09/02, 85-86. Bardin, M.; Dogimont, C.; Nicot, P.; Pitrat, M., 1999: Genetic analysis of resistance of melon line PI 124112 to Sphaerotheca fuliginea and Erysiphe cichoracearum studied in recombinant inbred lines, Abak, K. (ed.); Buyukalaca, S. (ed.); ISHS; International Society for Horticultural Science; Louvain (BEL); Cucurbits; Acta Horticulturae (NLD) 1. International Symposium; Adana (TUR); 1997/05/20-23, no. 492; 163-168, ISHS; Louvain (BEL). Blancard, D.; Pitrat, M.; Jourdain, F., 1989: Etude de la sporulation de Pseudoperonospora cubensis (Berk. et Curt.) Rost. sur cotylédons de melon, application à la recherche de variétés résistantes, Phytopathologia Mediterranea (ITA), no. 28; 169-175. Dogimont, C., 1995: Résistance du melon aux oïdiums des cucurbitacées. Présentation du Club Mildew, INRA; Centre de Recherche d'Avignon; Station de Pathologie Végétale; Montfavet (FRA); Compte-rendu, 4. Réunion du Groupe oïdium; Avignon (FRA); 1995/04/25-26, 5 p., INRA; Avignon (FRA). Dogimont, C.; Bordat, D.; Pagès, C.; Boissot, N.; Pitrat, M., 1999: One dominant gene conferring the resistance to the leafminer, Liriomyza trifolii (Burgess) diptera: Agromyzidae in melon (Cucumis melo L.), Euphytica (NLD), vol. 105; 63-67. Dogimont, C.; Bordat, D.; Pitrat, M.; Pagès, C., 1995: Characterization of resistance to Liriomyza trifolii (Burgess) in melon (Cucumis melo L.), Fruits (FRA), vol. 50 no. 6; 449-452. Dogimont, C.; Bordat, D.; Pitrat, M.; Pages, C., 1994: Mise en évidence d'une résistance à Liriomyza trifolii (Burgess) chez le melon (Cucumis melo L.), CIRAD; Centre de Coopération Internationale en Recherche Agronomique pour le Développement; Département des Productions Fruitières et Horticoles; Montpellier (FRA); Réunion annuelle 1994. Programme et résumés des communications, Productions horticoles; Montpellier (FRA); 1994/08/29; 1994/09/02, 1 p., CIRAD; Montpellier (FRA). Dogimont, C.; Thabuis, A.; Pitrat, M.; Lecoq, H., 1999: Différentes résistances au cucurbit aphid borne yellows luteovirus chez le melon contrôlées par deux gènes récessifs complémentaires, Yot, P. (ed.); CNRS; Département des Sciences de la Vie; Paris (FRA); INRA; Département Santé des Plantes et Environnement; Paris (FRA); CIRAD; Centre de Coopération Internationale en Recherche Agronomique pour le Développement; Délégation Scientifique Défense des Cultures; Montpellier (FRA); SFP; Société Française de Phytopathologie; Le Rheu (FRA); Virologie végétale, 7. Rencontres; Aussois, (FRA); 1999/03/14-18, 49. Dogimont, C.; Bussemakers, A.; Martin, J.; Slama, S.; Lecoq, H.; Pitrat, M., 1997: Two complementary recessive genes conferring resistance to cucurbit aphid borne yellows luteovirus in an indian melon line (Cucumis melo L.), Euphytica (NLD), no. 96; 391-395. Dogimont, C.; Bussemakers, A.; Slama, S.; Martin, J.; Lecoq, H.; Pitrat, M., 1996: Diversity of resistance sources to cucurbit aphid borne yellows luteovirus in melon and genetics of resistance, CPVO-TP/104/2 Final Date: 21/03/2007 EUCARPIA; European Association for Research on Plant Breeding; Paris (FRA); Cucurbits towards 2000, 5. Eucarpia Meeting on Cucurbit Genetics and Breeding; Malaga (ESP); 1996/05/28-30, 328-333, EUCARPIA; Paris (FRA). Dogimont, C.; Slama, S.; Martin, J.; Lecoq, H.; Pitrat, M., 1996: Sources of resistance to cucurbit aphid borne yellows luteovirus in a melon germ plasm collection, Plant Disease (USA), vol. 80 no. 2; 1379-1382. Dogimont, C.; Slama, S.; Martin, J.; Lecoq, H.; Pitrat, M., 1995: A la recherche de résistances au Cucurbit aphid borne yellows virus chez le melon, INRA; Institut National de la Recherche Agronomique; Paris (FRA); CNRS; Centre National de la Recherche Scientifique; Paris (FRA); Rencontres de Virologie végétale, 5; Aussois (FRA); 1995/01/23-27, 39, CNRS; Paris (FRA). Epinat, C.; Pitrat, M.; Bertrand, F., 1993: Genetic analysis of resistance of five melon lines to powdery mildews, Euphytica (NLD), no. 65; 135-144. Hosoya, K.; Narisawa, K.; Pitrat, M.; Ezura, H., 1999: Race identification in powdery mildew (Sphaerotheca fuliginea) on melon (Cucumis melo) in Japan, Plant Breeding (DEU), no. 118; 259-262. Lecoq, H.; Pitrat, M.; Bon, M.; Wipf Scheibel, C.; Bourdin, D., 1992: Resistance in melon to cucurbit aphid borne yellows virus, a luteovirus infecting cucurbits, 5. EUCARPIA Cucurbitaceae Symposium; Skierniewice (POL); 1992/07/27-31, 191-196, Research Institute of Vegetable Crops; Skierniewice (POL). Mahgoub, H.A.; Wipf-Scheibel, C.; Delécolle, B.; Pitrat, M.; Dafalla, G.; Lecoq, H., 1997: Melon rugose mosaic virus: characterization of an isolate from Sudan and seed transmission in melon, Plant Disease (USA), vol. 81 no. 6; 656-660. Morris, C.; Pitrat, M., 1998: La bactériose du melon: Connaissances acquises et travaux en cours, PHM Revue Horticole (FRA), no. 393; 44-47. Mc Creight, J.D.; Pitrat, M., 1993: Club mildew : working group on resistance of melon to powdery mildew, Cucurbit Genetics Cooperative (USA), no. 16; 39. Pitrat, M.; Dogimont, C.; Bardin, M., 1998: Resistance to fungal diseases of foliage in melon, Mc Creight, J.D. (ed.); ASHS; American Society for Horticultural Science; Alexandria (USA); Evaluation and enhancement of cucurbit germplasm, Cucurbitaceae ‘98; Pacific Grove (USA); 1998/11/30; 1998/12/04, 167-173, ASHS; Alexandria (USA). Pitrat, M.; Risser, G.; Bertrand, F; Blancard, D.; Lecoq, H., 1996: Evaluation of a melon collection for disease resistances, EUCARPIA; European Association for Research on Plant Breeding; Paris (FRA); Cucurbits towards 2000, 5. Eucarpia Meeting on Cucurbit Genetics and Breeding; Malaga (ESP); 1996/05/28-30, 49-58, EUCARPIA; Paris (FRA). Pitrat, M., 1996: Contrôle génétique des résistances aux maladies chez le melon, INRA; Direction des Relations Internationales; Secteur Méditerranée; Paris (FRA); IRTA; Institut de Recerca i Tecnologia Alimentaries; Barcelone (ESP); Lutte intégrée et exploitation de la diversité génétique chez les fruits et légumes, Séminaire INRA-IRTA; Barcelone (ESP); 1996/10/24-25, 44-51. CPVO-TP/104/2 Final Date: 21/03/2007 Pitrat, M., 1993: La lutte génétique, un moyen biologique de protection. Le point sur les résistances aux maladies chez le melon, Vaucluse Agricole (FRA), no. 1368; 9-10 Pochard, E.; Pitrat, M., 1990: Stratégie de lutte génétique contre les maladies à virus des plantes: exemple du melon et du piment en zone méditerranéenne, Sélectionneur Français (FRA), Parasites animaux et végétaux des cultures maraîchères de plein champ, et méthodes de lutte; Siracusa (ITA); 1988/02/22-24, no. 41; 63-70. Pitrat, M.; Dogimont, C.; Hagen, L.; Burget, E.; Lecoq, H.; Bendahmane, A., 2001: La résistance du melon au puceron Aphis gossypii INRA Mensuel (FRA), no. 111; 17-19. Pitrat, M.; Lecoq, H.; Lapchin, L., 1995: Stabilité des résistances aux virus et au puceron Aphis gossypii chez le melon, INRA, CTPS Comité Scientifique, Paris (FRA); Etude de la co-évolution des populations végétales domestiques face à leurs agents pathogènes ou ravageurs Séminaire; Paris (FRA); 1995/06/21, 27-32. Pitrat, M.; Maestro, C.; Ferriere, C.; Ricard, M.; Alvarez, J., 1988: Resistance to Aphis gossypii in spanish melon (Cucumis melo), Cucurbit Genetics Cooperative (USA), vol. 11 no. 51: 2 p. Pitrat, M.; Lecoq, H., 1982: Relations génétiques entre les résistances par non acceptation et par antibiose du melon à Aphis gossypii. Recherche de liaisons avec d'autres gènes, 1982Agronomie (FRA), vol. 2 no. 6; 503-508. Pitrat, M.; Lecoq, H., 1980: Non acceptance of melon to Aphis gossypii, its inheritance and relation to antibiosis, tolerance and resistance to virus transmission, Resistance to insects and mites, 2. EUCARPIA/IOBC Meeting of the working group; Canterbury (GBR); 1980/04/09-11; 5 p. Pitrat, M.; Bordat, D.; Dalle, M., 1993: Recherche de résistances chez le melon (Cucumis melo L.) envers Liriomyza trifolii (Burgess), Diptera Agromyzidae, CIRAD; Centre de Coopération Internationale en Recherche Agronomique pour le Développement; Mission de Coopération Phytosanitaire; Montpellier (FRA); Liriomyza, Colloque sur les mouches mineuses des plantes cultivées; Montpellier (FRA); 1993/03/24-26, 127-133, CIRAD; Montpellier (FRA). Pitrat, M.; Lecoq, H.; Wipf-Scheibel, C., 1993: Hérédité de la résistance du melon au cucurbit aphid borne yellows virus, INRA; Institut National de la Recherche Agronomique: Paris (FRA); CNRS; Centre National de la Recherche Scientifique; Paris (FRA); Résumés des communications, 4. Rencontres de virologie végétale; Aussois (FRA); 1993/01/25-29, 16, CNRS; Aussois (FRA). Pitrat, M., 1997: Melon: les résistances aux virus, Fruits et Légumes (FRA), no. 151: 15. Lecoq, H.; Clauzel, J.M.; Pitrat, M., 1989: Epidémiologies comparées du CMV, du WMV2, du ZYMV, et du PRSV chez des variétés de melon sensible ou possédant des résistances partielles, CNRS; Centre National de la Recherche Scientifique; Paris (FRA; INRA; Institut National de la Recherche Agronomique; Paris (FRA); Secondes rencontres de virologie végétale, 2. Rencontres; Aussois (FRA); 1989/01/24-28, 14, CNRS; Paris (FRA). Pitrat, M.; Lecoq, H., 1984: Exploitation de différentes formes de résistance aux virus chez le melon, Sélectionneur Français (FRA), Journée ASF; Versailles (FRA); 1984/02/02, no. 34; 29-37. CPVO-TP/104/2 Final Date: 21/03/2007 Pitrat, M.; Blancard, D., 1988: Le mildiou du melon (variétés résistantes et méthodes de lutte). Rapport final 1988, 4 p. INRA; GAFL; Génétique et Amélioration des Fruits et Légumes; Centre de recherche d'Avignon (FRA). Pochard, E.; Pitrat, M., 1988: Stratégie de lutte génétique contre les maladies à virus des plantes: exemple du melon et du piment en zone méditerranéenne, Parasites animaux et végétaux des cultures maraîchères de plein champ et méthodes de lutte, Congres; Siracusa (ITA); 1988/02/22-24, 6 p., Association phytopathologique italienne (ITA). Taha Yousif, M; Khey-Pour, A; Gronenborn, B.; Pitrat, M.; Dogimont, C., 2001 : Recherche de sources de résistance au watermelon chlorotic stunt begomovirus (WMCSV) chez le melon (Cucumis melo L.) et hérédité de la résistance, INRA; Paris (FRA); CNRS; Paris (FRA); CIRAD; Centre de Coopération Internationale en Recherche Agronomique pour le Développement; Montpellier (FRA); Virologie végétale, 8. Rencontres; Aussois, (FRA); 2001/03/11-15, 33. CPVO-TP/104/2 Final Date: 21/03/2007 Community Plant Variety Office
TECHNICAL QUESTIONNAIRE
to be completed in connection with an application for Community Plant Variety Rights Please answer all questions. A question without any answer will lead to a non-attribution of an application date. In cases where a field / question is not applicable, please state so. Botanical taxon: Name of the genus, species or sub-species to which the variety belongs and
common name

Cucumis
melo
Applicant(s): Name(s) and address(es), phone and fax number(s), Email address, and where
appropriate name and address of the procedural representative
Variety denomination
a) Where appropriate proposal for a variety denomination: b) Provisional designation (breeder's reference): CPVO-TP/104/2 Final Date: 21/03/2007 Information on origin, maintenance and reproduction of the variety
Method of maintenance and reproduction
ii) open-pollinated variety iii) parent line (ii) seed propagated (iii) vegetatively propagated Other information on genetic origin and breeding method Geographical origin of the variety: the region and the country in which the variety was bred or
discovered and developed
Characteristics of the variety to be indicated (the number in brackets refers to the
corresponding characteristic in the CPVO Protocol; please mark the state of expression
which best corresponds).
Example varieties
Inflorescence: sex expression (at full flowering)
Alpha, Categoía Fruit : length
Doublon, Golden Crispy Topper, Védrantais Marina, Spanglia Categoría, Toledo Katsura Giant, Valdivia CPVO-TP/104/2 Final Date: 21/03/2007 Example varieties
Fruit: shape in longitudinal section
De Cavaillon, Piolín Jívaro, Noir de Carmes Fruit: ground colour of skin
Albino, Honey Dew Amarillo-Canario, Edén, Galia, Passport, Gohyang, Piel de Sapo Geaprince, Geamar, Romeo, Sirio, Supporter, Védrantais Fruit: density of patches
absent or very sparse Fruit: grooves
absent or very weakly expressed Piel de Sapo, Arava weakly expressed strongly expressed Védrantais, Galia CPVO-TP/104/2 Final Date: 21/03/2007 Example varieties
Fruit: cork formation
Fruit: pattern of cork formation
Hermes, Védrantrais linear and netted Fruit: main colour of flesh
Seed: length
Geumssaraki, Golden Crispi Elario, Katsura Giant Amarillo Oro, Toledo Seed: colour
Amarillo Oro s.b. Galia, Piel de Sapo CPVO-TP/104/2 Final Date: 21/03/2007 Example varieties
Resistance to Fusarium oxysporum f. sp. melonis Race 0
Jador, Joker, Védrantais Resistance to Fusarium oxysporum f. sp. melonis Race 1
Jaune Canari 2, Védrantais Resistance to Fusarium oxysporum f. sp. melonis Race 2
Jaune Canari 2, Joker Jador, Védrantais Similar varieties and differences from these varieties:
Characteristic in which the State of expression State of expression of similar variety is different1) of similar variety candidate variety In the case of identical states of expressions of both varieties, please indicate the size of the difference CPVO-TP/104/2 Final Date: 21/03/2007 Additional information which may help to distinguish the variety
Applicants are requested to provide on a voluntary basis together with the Technical Questionnaire, representative printed-out colour photos of the candidate variety. Resistance to pests and diseases
absent present not tested Resistance to Fusarium oxysporum f. sp melonis a) Race 1-2 (char. 68.4) Resistance to Sphaerotheca flilginea (Podosphaera xanthii) (Powdery mildew) a) Race 1 (char. 69.1) c) Race 2 (char 69.2) d) Race 5 (char. 69.3) Resistance to Erysiphe cichoracearum Race 1 (Powdery mildew) (Golovinomyces cichoracearum) (char. 70) Resistance to colonisation by Aphis gossypii (char. 71) Resistance to Zucchini Yellow Mosaic Virus (ZYMV) Race F (char. 72) Resistance to Papaya Ring Spot Virus (PRSV) a) Race GVA (char. 73.1) Resistance to Muskmelon Necrotic Spot Virus (MNSV) [ ] viii) Resistance to Cucumber Mosaic Virus (CMV) (char.7.5) [ ] Other resistances (specify) CPVO-TP/104/2 Final Date: 21/03/2007 Special conditions for the examination of the variety
[ ] YES, please specify 7.3 Other
information
[ ] YES, please specify 8. GMO-information
required
The variety represents a Genetically Modified Organism within the meaning of Article 2(2) of Council Directive 2001/18/EC of 12/03/2001. [ ] YES If yes, please add a copy of the written attestation of the responsible authorities stating that a technical examination of the variety under Articles 55 and 56 of the Basic Regulation 2100/94 does not pose risks to the environment according to the norms of the above-mentioned Directive. CPVO-TP/104/2 Final Date: 21/03/2007 Information on plant material to be examined
9.1 The expression of a characteristic or several characteristics of a variety may be affected
by factors, such as pests and disease, chemical treatment (e.g. growth retardants or
pesticides), effects of tissue culture, different rootstocks, scions taken from different growth
phases of a tree, etc.
9.2 The plant material should not have undergone any treatment which would affect the
expression of the characteristics of the variety, unless the competent authorities allow or
request such treatment. If the plant material has undergone such treatment, full details of the
treatment must be given. In this respect, please indicate below, to the best of your knowledge,
if the plant material to be examined has been subjected to:
(a) Microorgan (e.g. virus, bacteria, phytoplasma)
(b) Chemical treatment (e.g. growth retardant or pesticide) (c) Tissue culture (d) Other factors Please provide details of where you have indicated "Yes": I/we hereby declare that to the best of my/our knowledge the information given in this form is complete and correct. Date Signature [End of document]

Source: http://www.naktuinbouw.nl/sites/default/files/TP_104-2_CUCUMIS_MELO.pdf