ANSWERS FOR SCIENCE. KNOWLEDGE FOR LIFE.™ High Resolution Separation and Quantitation of Charged and Polar Analytes P/ACE™ MDQ PLUS CAPILLARY ELECTROPHORESIS SYSTEM

A Robust, Automated Analytical ToolSCIEX Capil ary Electrophoresis (CE) has global y proven itself in many different research projects and industrial applications, including biological science, chemistry, forensic science, food and beverage, petrochemical, biofuels, environmental safety and more… This can be attributed to its simplicity, accuracy, robustness, speed, versatility, and micro sample capabilities, combined with low operating costs and minimal waste production.
1. Pheniramine2. Chlorpheniramine Separation and matrix challenges can be addressed 3. Brompheniramine4. Anileridine by a number of CE techniques: 6. Methamphetamine 7. Trifluoperazine 8. Pseudoephedrine Capil ary Zone Electrophoresis (CZE) 10. Methoxamine (IS) 11. Diphenhydramine Capillary Gel Electrophoresis (CGE) 12. Dextromethorphan 14. Hydroxyzine15. Salbutamol (ES) Capil ary Isoelectric Focusing (CIEF) Absorbance @ 200nm 17. Trazodone18. Haloperidol Isotachophoresis (ITP) 19. Verapamil20. Loperamide Micellar Electrokinetic Capil ary Chromatography (MECC) Capillary Electrochromatography (CEC) Figure 1: Highly resolved separation of 20 basic drugs using low pH, where the capillary surface is essentially neutral and the amine functional groups Non Aqueous Capil ary Electrophoresis (NACE) on the solutes are maximally ionized For Research Use Only. Not for use in diagnostic procedures.

P/ACE™ MDQ Plus Capillary Electrophoresis System Built-in auto-sampler and programmable array of 36 pairs of buffers. Exclusive circulating liquid, precisely regulates capillary temperature Sample temperature control Integrated interchangeable detector modules Multi-mode sample introduction Internal fluidic system generates pressure or vacuum Sample from vials, micro vials or 96-well plates Fully programmable separation methods For Research Use Only. Not for use in diagnostic procedures.

Our Customers Have Found Numerous Methods and Applications in Academic Research, Industry P/ACE™ MDQ Plus Forensics / Drug Screening Food and Beverage Manufacturing While the highly polar nature of basic The P/ACE MDQ Plus, provides in-depth analysis pharmaceuticals makes Liquid Chromatography of food compounds including anions, cations, complex, CE has the ability to excel at such organic acid content, and protein integrity, to better manage product process consistency, taste, The robust nature of this tool and the ruggedness quality, storage conditions, safety, adulteration of this particular approach are appropriate for and authentication. screening protocols used in forensic toxicology.
1. Pheniramine2. Chlorpheniramine3. Brompheniramine 6. Methamphetamine White Wine Analysis 7. Trifluoperazine 8. Pseudoephedrine 10. Methoxamine (IS) 11. Diphenhydramine 12. Dextromethorphan 15. Salbutamol (ES) Absorbance @ 200nm 100 mM phosphate pH 2.38 Figure 2: Highly resolved separation of 20 basic drugs using low pH, where
Figure 3: Analysis of white wine, revealing quantifiable compounds that
the capillary surface is essentially neutral and the amine functional groups effect the taste and feel. on the solutes are maximally ionized For Research Use Only. Not for use in diagnostic procedures.
Water Quality and Environmental Analysis of anion and cation content gives you a The P/ACE MDQ Plus already excels in ion analysis With ever-increasing water demands, quick and better understanding of the chemical makeup of and is a natural fit for biofuel production. The efficient identification of impurities, analytes or other petroleum and related products. For example highly fermentation of lignocellulosic biomass to ethanol is contaminating molecules within a water sample is a purified terephthalic acid (PTA) and its esters are an attractive route to fuels to supplement fossil fuels. key step in managing the safety of our water supply.
major components in the production of plastics and Incorporating ion analysis and sugar analysis makes Water is also a vital component in industrial fibers like polyethylene glycol and polyester. The the P/ACE MDQ Plus invaluable for monitoring the production of semiconductors, metal plating, and purity of PTA has a direct effect on the quality and fermentation and purification processes used in the is used in large volumes in nuclear power plants color of the end products. production of ethanol. and pulp and per mills. The P/ACE MDQ Plus identifies analytes that lead to corrosion as well as contaminants that contribute to downstream environmental pollution. Terephthalic Acid Characterization of Biofuel Production 2. Coniferyl aldehyde 3. Acetoguaiacone 4. Syringaldehyde 7. p-coumaric acid 10. 4-hydroxybenzoic acid Figure 4: With the P/ACE MDQ Plus, sensitive, reproducible, high resolution
Figure 5: CE Analysis of lignocellulosic biomass derived organic acids.
Figure 6: Separation performance of the P/ACE MDQ Plus in under 5 min
separations are achieved quickly, characterizing PTA and its major impurities.
-Turkia et al. J Chrom A, 2013 demonstrating use in identifying Inorganic Anions in water.
For Research Use Only. Not for use in diagnostic procedures.

A Robust Solution for Demanding Analytical Requirements Automated Sample Handling Quick-Change Electrodes The P/ACE MDQ Plus system offers fully System electrodes and vial cap openers simplify automated methods and extended sample- user interaction, are designed to assure high handling capability for walkaway operation. strength and rigidity and are easily removed for maintenance.
Sampling can be performed using 1.8 ml universal vials, 96-well plates and micro vials.
Universal Vial and Cap Design Precision-molded polymethylpentene universal Prevents the capillary and electrode from vials accommodate run buffer, sample and physically interacting with the caps, creates a better seal, ultimately allowing for a robust, clean sample interface.
For Research Use Only. Not for use in diagnostic procedures.
With Built-in: – Auto-sampler – Pressure and Vacuum – Sample and Capillary Temperature Control Multiple Modes of Sample Introduction Sample Temperature Control Settable temperature between 4 - 60°C helps maintain Capillary electrophoresis separation coupled to The P/ACE MDQ Plus provides electrokinetic, pressure sample stability or perform functional assays mass spectrometry or ultra-low flow connection and vacuum introduction of samples. Introduction from to mass spectrometry (MS) combines the high- either end of the capillary allows both ultrafast and Variable Pressure and Vacuum resolution separation of CE and the high-sensitivity high-resolution analyses The internal fluidic system operates with all common mass determination of MS. The P/ACE MDQ Plus rinsing protocols, regulating these with a pressure- accommodates direct MS connection through the • Separations can be adjusted by varying voltage, current, pressure and vacuum handling capability of -5 to 100 p.s.i.
right-side access panel. Capillary temperature control is maintained. Capillary conditioning is accomplished by moving • Combination of voltage and pressure ensures the buffer stays free of air bubbles which can be specific volumes of electrolytes, gels, regenerants generated from outgassing and cleaning solutions through capillary Gel buffers are quickly and efficiently pumped into capillary Capillary Temperature Control Efficient CE separations rely on precise capillary temperature regulation to manage Joule heating within the capillary. Proper temperature control plays important role in repeatability of analysis Recirculating liquid coolant provides effective capil ary temperature control Improved migration time reproducibility Use of high ionic strength buffers for optimal resolution of complex mixtures Use of large bore capil aries to accommodate larger mass loads and enhance sensitivity Capil ary temperature can be regulated between 15 - 60°C.
Capil aries are housed in cartridges facilitating temperature control and easy exchange of capil ary dimensions and surfaces.
For Research Use Only. Not for use in diagnostic procedures.
Integrated Detector ModulesThe Ultimate in Versatility A Modular Approach Supports flexible research, methods development and The P/ACE MDQ Plus is easily configured to run a wide rugged routine use. Instrument design makes it easy to variety of procedures, fast and efficiently, adapting to interchange high-sensitivity diode array (DAD), UV/Vis, specific demands for a plethora of applications. and laser induced fluorescence (LIF) modules. External detector adapter allows the capillary to be extended to additional detection systems. Solid State 488 nm UV/Vis Detection Module Photodiode Array Detection Module Provides absorbance spectroscopy in the UV-visible Lets you preform high-sensitivity analysis across a And laser-induced fluorescence (LIF) detector provide region. Commonly used exclusion filters at 200, 214, broad range of wavelengths. Allows for spectral robust high-sensitivity analysis of labeled molecular 230, and 254 nm are provided to increase analyte wavelength analysis between 190 and 600 nm species. Quiet, energy-efficient compact design specificity. Important when using photosensitive with baseline subtraction.
reduces overall system footprint. Two-color excitation capillary surfaces.
and dual wavelength detector may be added.
For Research Use Only. Not for use in diagnostic procedures.
Working in Conjunction with P/ACE MDQ HardwareOur 32 Karat software – unique to capillary electrophoresis – provides instrument control, data acquisition and analysis.
Control and Analysis Methods Development With direct control, you get intuitive management Methods are defined and edited in table format. An array of up to 36 pairs of buffers provides the of all system functions and ability to set run All functions for the system are handled in a flexibility for the rapid optimization of methods parameters in real time. single window, including programming of the development. Automates the method development buffer array for the automation of methods process by implementing strategies that analyzes Velocity-Calibrated Peak Area and CAESAR development strategies. your samples using an array of buffers with different integration ensure reproducible quantitation pH levels, ionic strength or additives. at low limits of detection All buffer information – including descriptions and/or preparation protocols can be stored Automated fractionation of detected peak with the method. allows isolation of newly resolved compounds for external identification In addition to automated peak identification, mobility plots allow you to assess physical molecular interactions and simplify interpretation by removing EOF variability.
Methoxamine IS 0.00020720 Diphenhydramine 1.990 0.00019868 oxyzine 1.792 0.00017986 Chlorpheniramine 3.081 0.00030731 ompheniramine 3.034 0.00030311 omethorphan 1.949 0.00019424 olol 1.666 0.00016624 Pheniramine 3.280 0.00032965 Pentazocine 1.703 0.00017039 Amphetamine 2.597 0.00025823 Haloperidol 1.517 0.00015386 Verapamil 1.391 0.00013891 Methamphetamine 2.515 0.00025063 Psuedoephedrine 2.330 0.00023278 Loperamide 1.330 0.00013222 Ephedrine 2.292 0.00022837 Codeine 1.871 0.00018703 Trazodone 1.566 0.00015680 Figure 7: Advanced Peak Identification uses mobility or a powerful
Figure 8: Buffer trays may be annotated with full descriptions –
Figure 9: Direct Control – A graphical interface to select and change
2D algorithm to couple mobility with spectral signature for the including buffer preparation protocols and saved as part of operating parameters with immediate real time execution and ultimate in peak identification.
For Research Use Only. Not for use in diagnostic procedures.
Supplies and Resources
Items can be ordered at
Supplies and Accessories Anion Analysis Kit 200 μL Microvials (pkg. of 100) Cation Analysis Kit Universal Vial Caps Methods Development Kit Electrode Replacement Kit LIFLUOR dsDNA 1000 Enhance Buffer Vial Tray (36 vials) Buffer Vial Tray (48 vials) Cartridge Assembly, 30 cm Capillary Capillary Test Kit Blank Cartridge Assembly Kit Cartridge Rebuilding Kit Cartridge Tubing Kit Capillary Coolant (450 mL) For Research Use Only. Not for use in diagnostic procedures.
System Specifications Ordering Information B52521 P/ACE MDQ Plus
Height: 29.2 in (74.2 cm) 2 x 96 well plates Capillary Electrophoresis System
Door Open: 38.8 in (98.6 cm) 2 x 48 universal vials Includes separation module and UV/Vis Width: 25 in (63.5 cm) 2 x 48 0.2 mL microvials detector module; system controller Depth: 28.4 in (72.1 cm) (Win 7) with 32 Karat software and Buffer Tray:
system start up kit.
2 x 36 universal vials 188 lbs (85.3 kg) B68372 Diode Array Detector Module
(includes UV/Vis Detector) Includes DAD detector module, Detection Capability optics cable, and board.
Voltage: 100 - 240 V; 50/60 Hz 200, 214, 230 and 254 nm filters B67326 Solid State Laser and LIF
190 - 600 nm (custom filter option) detector module, 488nm.
1 to 30 kV programmable Includes optics cable, solid state laser at 0.1 kV increments Diode Array (optional)
and single color detector module.
190 - 600 nm programmable 0.5 - 32 Hz scan collection frequency B67327 LIF 2 Color Upgrade Kit
3 to 300 μA programmable Converts LIF detector to dual- at 0.1 μA increments wavelength optics. Laser Induced Fluorescence (LIF) (optional)
Pressure Delivery Range:
300 - 700 nm excitation range 350 - 750 nm emission range0 - 1000 RFU Sample Temperature Control:
Source Lasers with 3 mW Power Output:
488 nm solid-state laser (included in B67326) Capillary Temperature Control:
For Research Use Only. Not for use in diagnostic procedures.
Your Success is Our SuccessWe take it personally As a SCIEX customer you have access to a world-class customer support organization. Wherever you are, we're there with you as a trusted partner to answer questions, provide solutions, and maximize lab productivity.
Our service engineers have the experience and expertise to help you get the most from your LC, CE and MS systems. Whether you're looking to improve sensitivity, resolution, speed or throughput, they can direct you to the right solution. 2015 AB Sciex. For Research Use Only. Not for use in diagnostic When you have questions, we have answers.
procedures. SCIEX is part of AB SCIEX. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. RUO-MKT-03-1992 2/2015 Learn more at Headquarters
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Supporting people with dementia and theircarers in health and social care Issued: November 2006 NICE clinical guideline NICE has accredited the process used by the Centre for Clinical Practice at NICE to produceguidelines. Accreditation is valid for 5 years from September 2009 and applies to guidelines producedsince April 2007 using the processes described in NICE's 'The guidelines manual' (2007, updated2009). More information on accreditation can be viewed at

Op-brai150415 1.15

Brain Advance Access published January 25, 2016 BRAIN 2016: Page 1 of 15 The neural dynamics of reward value and riskcoding in the human orbitofrontal cortex Yansong Li,1,2, Giovanna Vanni-Mercier,1,2 Jean Isnard,2,3 Franc¸ois Mauguie re2,3 andJean-Claude Dreher1,2 See Kringelbach (doi10.1093/awwxxx) for a scientific commentary on this article. The orbitofrontal cortex is known to carry information regarding expected reward, risk and experienced outcome. Yet, due toinherent limitations in lesion and neuroimaging methods, the neural dynamics of these computations has remained elusive inhumans. Here, taking advantage of the high temporal definition of intracranial recordings, we characterize the neurophysiologicalsignatures of the intact orbitofrontal cortex in processing information relevant for risky decisions. Local field potentials were

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