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Pearl.elte.huEur. J. Biochem. 270, 3109–3121 (2003) FEBS 2003 Gene regulation by tetracyclinesConstraints of resistance regulation in bacteria shape TetR for applicationin eukaryotes Christian Berens and Wolfgang Hillen Lehrstuhl fu¨r Mikrobiologie, Institut fu¨r Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander Universita¨t Erlangen-Nu¨rnberg;Germany The Tet repressor protein (TetR) regulates transcription are fused to TetR to turn it into an eﬃcient regulator.
of a family of tetracycline (tc) resistance determinants in Mechanistic understanding and the ability to engineer and Gram-negative bacteria. The resistance protein TetA, a screen for mutants with speciﬁc properties allow tailoring membrane-spanning H+-[tcÆM]+ antiporter, must be sen- of the DNA recognition speciﬁcity, the response to inducer sitively regulated because its expression is harmful in the tc and the dimerization speciﬁcity of TetR-based eukary- absence of tc, yet it has to be expressed before the drugs' otic regulators. This review provides an overview of the concentration reaches cytoplasmic levels inhibitory for TetR properties as they evolved in bacteria, the functional protein synthesis. Consequently, TetR shows highly speci- modiﬁcations necessary to transform it into a convenient, ﬁc tetO binding to reduce basal expression and high aﬃnity speciﬁc and eﬃcient regulator for use in eukaryotes and to tc to ensure sensitive induction. Tc can cross biological how the interplay between structure ) function studies in membranes by diﬀusion enabling this inducer to penetrate bacteria and speciﬁc requirements of particular applica- the majority of cells. These regulatory and pharmacological tions in eukaryotes have made it a versatile and highly properties are the basis for application of TetR to selec- adaptable regulatory system.
tively control the expression of single genes in lower and Keywords: antibiotic resistance; disease models; fusion pro- higher eukaryotes. TetR can be used for that purpose in tein; inducible gene expression; ligand-binding speciﬁcity; some organisms without further modiﬁcations. In mam- mammalian cell lines; protein engineering; structure–activity mals and in a large variety of other organisms, however, relationship; Tet repressor; transgenic organism.
eukaryotic transcriptional activator or repressor domains Properties of bacterial Tet systems of transcription by the tc-responsive Tet repressor (TetR).
In the absence of inducer, TetR dimers bind to the operators Efflux-mediated tetracycline resistance is always tetO1 and tetO2, shutting down transcription of its own regulated in Gram-negative bacteria gene, tetR, and of the resistance gene, tetA. Once tc hasentered the cell, it binds TetR with high afﬁnity as a In Gram-negative bacteria, resistance to tetracyclines (tc) [tcÆMg]+ complex . This induces a conformational change is mediated by the TetA protein, a proton-[tcÆMg]+ anti- in TetR  resulting in dissociation from tetO . The porter embedded in the cytoplasmic membrane [1,2]. Eleven following expression burst of TetA and TetR leads to a tc resistance determinants (Tet classes A–E, G, H, J, Z, 30, rapid reduction of the cytoplasmic tc concentration  and 33 [3–5]) share the organization of structural and which, in turn, shuts expression of both genes off again.
regulatory genes (reviewed in ). In enteric bacteria, the Expression of TetA is ﬁne-tuned in the presence of tc so that efﬂux-encoding tetA genes are strictly regulated at the level export overcomes the slow uptake (compare below).
Regulation of Tc resistance is optimized for tightness Correspondence to W. Hillen, Lehrstuhl fu¨r Mikrobiologie, Institut fu¨r Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander Regulation of tet determinants is subject to strong, opposing Universita¨t, Staudtstr. 5, D-91058 Erlangen, Germany.
selective pressures. Expression of the resistance protein Fax: +49 9131 8528082, Tel.: +49 9131 8528081,E-mail: firstname.lastname@example.org TetA is detrimental to the cell [11,12]. Overexpression of this Abbreviations: tc, tetracycline; dox, doxycycline; atc, anhydrotetra- integral membrane protein is lethal for Escherichia coli , cycline; tTA, tetracycline-dependent transactivator; rtTA, reverse probably due to the collapse of the membrane potential .
tetracycline-dependent transactivator; tTS, tetracycline-dependent Consequently, expression of TetA must be tightly repressed trans-silencer; CMV, cytomegalovirus; GFP, green ﬂuorescent in the absence of the drug. However, when tc diffuses into the cell the resistance protein must be expressed before the (Received 8 April 2003, revised 14 May 2003, cytoplasmic concentration of tc reaches the micromolar accepted 15 May 2003) level necessary to inhibit translation. This requires: (a) high 3110 C. Berens and W. Hillen (Eur. J. Biochem. 270) competing nonspeciﬁc DNA to a much higher degree thanbacteria. Taken together, the evolutionary pressures ontc-dependent gene regulation have led to tight repression inthe absence of tc, without compromising sensitivity ofinduction, so that regulated tc resistance determinantsimpose no burden on the ﬁtness of E . coli in the absence ofthe antibiotic, but still mediate high levels of resistance to tcin its presence .
The structural change of TetR associated with inductionby tetracycline is known X-ray crystal structures of free TetR , TetR complexedwith different tetracyclines [18–21] and with tetO  havebeen determined at resolutions of 1.9–2.5 A˚, revealingthe allosteric conformational change leading to induction.
These results have been reviewed in detail  and have beencompared to Lac repressor . Thus, they are onlysummarized here (Fig. 2). The DNA reading head of TetR(magenta) is connected to the protein core (blue) by the helixa4 (green). Binding of [tcÆMg]+ (yellow) to TetR unwindsthe C-terminal residues of helix a6 (light blue), which bumpinto a4 and displace it. As the C terminus of a4 is held inplace by contacts to tc, the displacement leads to apendulum-like swing of the a4 N terminus increasing thedistance between the recognition helices by 3 A˚, so that theydo not ﬁt into successive major grooves of DNA anymore. These conformational changes are consistent withmany noninducible TetR mutants [24,25], spectroscopicanalysis of TetR in vitro , in vivo  and in vitro disulﬁde trapping experiments. Furthermore, a movementof a9 closes the tc binding pocket after the drug has entered, and the loop between a8 and a9 is also important for Fig. 1. Structures of tetracyclines used in eukaryotic gene regulation.
(A) Structure of tetracycline with the pKa values of the three titratablegroups. (B) Structure of doxycycline. (C) Structure of anhydrotetra-cycline.
Tetracycline penetrates cells by diffusion Tetracyclines (Fig. 1) diffuse in their uncharged forms afﬁnity of TetR for both tetO and tc to keep the basal through lipid bilayers without the aid of protein channels expression level of tetA low and to ensure that its [32–36]. Measuring the increase in ﬂuorescence intensity of transcription is initiated at concentrations which are still tc observed upon binding to TetR  allows us to determine subinhibitory for translation; (b) low afﬁnity of the TetR– the cytoplasmic concentration of tc and, thus, to calculate [tcÆMg]+ complex for DNA; and (c) high-level, but short- permeation coefﬁcients for tc uptake into liposomes term expression of TetA to initially reduce the internal [(2.4 ± 0.6) · 10)9 cmÆs)1] concentration of tc. A low level of TetR is important for [(5.6 ± 1.9) · 10)9 cmÆs)1] . These translate into half- sensitive induction, since E . coli strains expressing high equilibration times of 35 ± 15 min for tc to cross the levels of TetR need high concentrations of tc for full membranes and are in good agreement with the half- induction . These conﬂicting requirements are met by equilibration time of 15 min measured for [3H]tc-uptake in the genetic organization of the resistance determinants Bacillus subtilis , and the slow uptake of tc observed in (reviewed in ) and by the ligand binding properties of Staphylococcus aureus . Tetracycline diffusion through TetR. High sensitivity towards tetracyclines [see Fig. 1 for phospholipid membranes is, thus, slow and appears to be the structures of tc, doxycycline (dox) and anhydro-tc (atc)] the rate-limiting step of uptake into cells . The previously is achieved by the remarkable binding constant of TetR for observed rapid uptake of tc [33,39] might rather reﬂect ), [doxÆMg]+ (Ka 1010 M ) or unspeciﬁc adsorption of tc to membrane surfaces [32,36]. A ) [7,9], about 103)105-fold higher detailed model explaining the transport and accumulation than the afﬁnity of the drugs to their intracellular target, the of tc across the Gram-negative cell envelope has been ribosome . Binding of two molecules of [tcÆMg]+ to a presented by Nikaido and coworkers ([40,41] and references TetR dimer diminishes repressor afﬁnity for tetO by about cited therein). In the medium, as well as in the periplasm and nine orders of magnitude to the unusually low background cytoplasm, tc is present in one uncharged and several DNA binding level of less than 105 . This high ratio charged or zwitterionic species, due to its three titratable of speciﬁc over nonspeciﬁc DNA binding enables TetR to groups (Fig. 1). The distribution between these species bind tetO efﬁciently, even in larger genomes containing depends on the pH of the respective compartment . The Gene regulation by tetracyclines (Eur. J. Biochem. 270) 3111 Fig. 2. Structure of the TetR–[tcÆMg]+complex. Tet repressor is shown as a ribbondiagram with one monomer in gray and theother monomer color-coded as follows: TheDNA-binding region is in magenta, the helixconnecting it with the protein core is in green.
The protein core is dark blue, with the helix a6in light blue. Tetracycline is displayed asspace-ﬁlling CPK model in yellow. For clarity,the helices a1–a10 of one monomer are num-bered and the N and C termini of both sub-units are indicated. The coordinates weretaken from the PDB entry 2TRT .
uncharged form of tc can penetrate the outer membrane repression by unmodiﬁed TetR . Here, TetR most directly. But the major fraction of tc equilibrates as a likely acts by interfering sterically with binding of RNA [tcÆM]+-complex rapidly through the outer membrane via polymerase or auxiliary transcription factors [42,54]. To porins, with the Donnan potential across the outer mem- achieve this, one or more tetO elements are placed in brane leading to a two- to threefold accumulation of this proximity of either the TATA box or the transcriptional charged complex in the periplasm. Tc then diffuses passively start site of the respective target gene and TetR is expressed in its uncharged form through the cytoplasmic membrane.
concomitantly by a strong, constitutive promoter. Promot- Due to the pH gradient across the cytoplasmic membrane, ers of all three eukaryotic RNA polymerases have been a larger fraction of the uncharged tc dissociates in the targeted in the manner described. Unfortunately, as will cytoplasm than in the periplasm. Since equilibrium is become evident in the following paragraph, the published, reached when the concentration of uncharged tc is identical successful approaches do not yet allow formation of a in both compartments, this results in a higher intracellular simple strategy for establishing a TetR-repressed system, concentration of [tcÆM]+, the biologically active compound.
although they clearly point out that the positioning of the Again, accumulation of tc is the product of this passive tetO boxes is crucial for efﬁcient regulation.
equilibration across the inner membrane [40,41].
In Leishmania donovani, an RNA polymerase I promo- ter was brought under tc-control by placing a single tetO site Tc-based gene regulation functions in 2–24 bp upstream of the transcriptional start site , different setups in many eukaryotic systems whereas in Trypanosoma brucei at least one tetO elementhad to be inserted at a position +2 or )2 relativ e to the The evolved properties of TetR described above combined transcription start site of an RNA polymerase I-like with the favorable pharmacokinetics of tetracyclines and promoter . For RNA polymerase III-mediated tran- their long record of safe use in clinical practice make the Tet scription of suppressor tRNA genes, induction factors system a good candidate to fulﬁll the criteria that are required between two- to ﬁvefold were observed in Saccharomyces for an ideal transcriptional regulator in eukaryotic cells as cerevisiae, Dictyostelium discoideum and carrot protoplasts given by Saez and others [42,43]. Consequently, the past when tetO was introduced within 10 bp upstream of the 15 years have seen the broad application of tc-dependent transcriptional start site [57–59]. A regulated version of the regulatory systems, mainly in mammalian cell culture, but to human U6 snRNA promoter, also transcribed by RNA an increasing degree in transgenic organisms like plants, polymerase III, was developed by replacing sequences yeasts, protozoan parasites, slime molds, ﬂies, and rodents.
between the proximal sequence element and the transcrip- These topics have been extensively reviewed [42–52]. The tional start site with tetO . Flanking the TATA-box with following section presents an overview of the basic Tet two operators completely abolished transcriptional activity.
systems used to regulate gene expression in eukaryotes.
In contrast, introduction of a single tetO element affectedtranscription only slightly, but led to up to 25-foldrepression in the presence of TetR. A regulated U6 snRNA Gene regulation by TetR in eukaryotes promoter with a deﬁned expression window [61,62] would The most basic and ﬁrst published application of be a very powerful tool as this promoter is used to express tc-mediated gene regulation in eukaryotes is transcriptional the small interfering RNA  needed for silencing gene
3112 C. Berens and W. Hillen (Eur. J. Biochem. 270) expression by RNA interference . Repression of RNA magnitude can be reached with sensitive reporter genes like polymerase II promoters exerted by TetR is strongest in ﬁreﬂy luciferase . Luciferase activity is expressed within plants [65,66], mammalian cells  and fungi like Schizo- 4 h of removal of tc and about 20% of the steady-state level saccharomyces pombe [68,69] when multiple tet operators is reached after 12 h. While the use of a strong, constitutive are positioned within a region from 5 bp upstream to 35 bp promoter (CMV IE, EF-1a, Ubiquitin C) is common in cell downstream of the TATA-element. In contrast, placement culture applications, the use of tissue-speciﬁc promoters in of one to four tet operators immediately downstream of the transgenic animals provides spatial control to the Tet transcription initiation site has been shown to be most system, restricting expression of the Tet transregulator and, effective in the parasitic protozoa Entamoeba histolytica subsequently, the transgene to the desired tissue [84,85]. In [70,71], Toxoplasma gondii  and Giardia lamblia .
Drosophila, usage of the Gal4-UAS system to control Tettransregulator expression allows the generation of spatiallydelimited expression patterns by simple crossing with one of Gene regulation by TetR-based transregulators the many Gal4 driver lines available in the Drosophila While unmodiﬁed TetR acts as a transcriptional repressor in research community .
plants and lower eukaryotes, it can be, but not always is One concern has been the expression levels of Tet efﬁcient in mammalian cells [67,74]. A consistently func- transregulators as inﬂuenced by a potentially low mRNA tional version for yeasts, ﬂies and mammalian cell lines is stability or efﬁciency of translation. This was recently TetR fused to an eukaryotic regulatory domain, such as an addressed by generating a synthetic coding sequence for acidic activation domain (Fig. 3A; tTA or Tet-Off) [75–80] tetR. Potential splice donor and acceptor sites identiﬁed by or a repression domain (Fig. 3B; tTS) [81–83]. The trans- sequence analysis, several potential endonuclease cleavage activator tTA directs expression from a tc-dependent sites, and potential stable hairpin structures in the mRNA promoter that contains seven repeats of a tetO2 element were eliminated and human codon usage was used [87–90].
from the transposon Tn10. The palindromic centers of two The consequence of this optimization protocol is a higher adjacent operators are separated by 41 bp. This element is protein level in Drosophila, HeLa and HEK293 cells.
fused to a minimal promoter, typically derived from the Another concern voiced was that the CMV-derived human cytomegalovirus (CMV) immediate early promoter minimal promoter was not transcriptionally silent under all . When both components are stably integrated into experimental conditions [91–93]. This promoter leakiness proper chromosomal loci of mammalian cell lines, tran- can be caused by promoter-dependent or integration site- scription from the hybrid promoter is silent in the presence dependent effects and has been discussed in detail .
of more than 10 ngÆmL)1 dox. Removal of dox leads to Promoter-dependent leakiness has been addressed by the binding of tTA to tetO and subsequent activation of use of alternative minimal promoters [75,95,96]. In transient transcription. Regulatory factors of up to ﬁve orders of transfection experiments, these show lower basal activities Fig. 3. Regulation of gene expression by Tet transregulators. The promoter proximal tetO boxes are represented by black boxes. The transregulatorsare shown as follows: the DNA reading heads are in light gray, the inducer-binding and dimerization domain is in dark gray, activation domains areblack boxes, and the silencing domain is stippled. The conformational change leading to the loss of DNA-binding activity is pictured as a light graybox. High-level activated transcription is displayed by a bold arrow, low-level basal transcription by a dotted arrow. (A) tTA. (B) tTS. (C) rtTA.
Gene regulation by tetracyclines (Eur. J. Biochem. 270) 3113 than Ptet-1, but also do not reach its maximal activation tion can be used to ﬁnd solutions to some of the problems level. Thus, the regulatory window for target gene expres- and limitations that arise for Tet system applications in sion is shifted and expanded due to the stronger reduction of the basal activity. Integration site-dependent leakiness hasbeen attributed to enhancers located close to the integration Alterations of the activator domain of tTA site of the target gene construct. Besides screening additionalclones until one harboring the desired properties is found, Especially for gene therapy, concern about a viral protein is the problem has been approached by insulating Ptet-1 from often voiced, as humoral as well as cellular immune external activating signals through insertion of a chicken response against the VP16 protein has been found in herpes lysozyme matrix attachment region just upstream of Ptet-1 simplex infected humans [106–108]. Thus, immune res-  or by ﬂanking the target gene expression unit with either ponses against transactivators containing the VP16 domain chicken b-globin insulators  or SCS and SCS' boundary cannot be rigorously excluded, although they have not been elements from Drosophila .
observed so far in a mouse model using reverse tTA (rtTA; A different strategy was adopted by engineering a Tet-On) . Two solutions circumventing this concern tc-controlled trans-silencer protein . Fusion of the have been developed: (a) the VP16 domain has been KRAB domain of Kox1  to TetR yielded a hybrid replaced by three repeats of a minimal activation domain protein called tTS, that not only substantially repressed derived from a 12-amino acid activating patch of the VP16 basal transcription from Ptet-1 even if the tet operators were protein (tTA2 ); and (b) a variety of human activator located 3 kbp distant from the minimal promoter, but also domains from the acidic, glutamine-rich, serine/threonine- efﬁciently down-regulated gene expression from a CMV rich and proline-rich functional groups were tested for their enhancer-driven Ptet-1 . This strategy therefore appears ability to replace the VP16 domain. When fused to TetR, to be more versatile in coping with unwanted target gene only acidic activation domains were highly active [78–80].
expression than the promoter adaptation proposed above.
Minor activation was observed with the serine/threonine- In addition and in contrast to tTA, the tc-dependent rich domains from the transcription factors ITF-1, ITF-2, silencing of complex promoters offers the unique possibility and MTF-1. Transactivators with activation potentials of reversibly down-regulating the expression of cellular spanning more than three orders of magnitude have been genes on top of their normal regulation. The KRAB generated by combination of various minimal activation domain is inactive in S. cerevisiae and Drosophila where it domains (see above; ). They are attractive for combined was replaced with repression domains from the proteins knock-in/knock-out strategies to convey tissue-speciﬁc SSN6 , knirps, giant or dCtBP .
expression of the transactivator, while at the same time The expression of transfected genes can be rapidly inactivating expression of the genomic copy of the target repressed in mammalian cells by epigenetic mechanisms gene. Expression of the regulatory protein is then an . Although this transgene silencing is not speciﬁc for invariant function of the genomic locus and, if too high, can the Tet system, it is often observed for genes under tc lead to squelching . This can be addressed by control due to its frequent usage as conditional expression employing a transactivator with reduced activation poten- system. Approaches to achieve stable gene expression have tial as these are tolerated in the cell at higher concentrations been to: (a) screen many transfected clones; (b) the use of lentiviral vectors ; (c) replace the viral promoters thatdirect expression of the transregulators with promoters of Conversion of TetR to reverse TetR human origin ; (d) use chromatin insulator sequencesto protect transgene expression ; or (e) couple transgene Eukaryotic gene regulation by tTA shows a high dynamic expression to a selectable marker via an IRES element range and works consistently well, but has several practical  or by fusion of the transregulator with green drawbacks. Tc has to be continually present to keep ﬂuorescent protein (GFP) . Note that in this fusion expression of the gene of interest downregulated. Although protein GFP is connected to the DNA-binding domain of tc is not toxic at the levels utilized in gene regulation, TetR which can interfere with nonspeciﬁc DNA-binding prolonged exposure to the antibiotic is not always desirable activity of TetR at low levels of dox (see Fig. 2A in  in transgenic animals nor is it possible in gene therapy.
and ). The few published examples make it impossible Furthermore, induction of target genes is mostly slow as it to recommend one of the strategies for use in establishing requires removal of the drug from the culture or organism.
homogenous expression of transgenes, but silencing of To be able to control the time point of induction more transregulator expression is not completely suppressed by precisely, and since organisms are more easily saturated the use of lentiviral vectors  or insulator sequences with an effector than depleted of it [111,112], reverse TetR variants which bind tetO only in the presence of tc weresearched for and found (Fig. 3C). Screening in E . coli  Modifications of the Tet transregulators and in S. cerevisiae  revealed that a small number ofmutations in TetR can lead to that phenotype . Once The TetR–VP16 fusion works very well in many cases, but this was discovered, intensive screening led to rtTA alleles in may not be optimal for all applications. Structure–function which the initial disadvantages of occasional background studies based on powerful selection and screening systems in expression and low sensitivity for dox were eliminated .
E . coli [104,105] and in S. cerevisiae  have lead to a The rtTA-S2 allele was obtained by screening for reduced profound understanding of how DNA binding, inducer background expression and rtTA-M2 was the result of binding and dimerization function in TetR. This informa- screening for higher sensitivity towards dox starting from 3114 C. Berens and W. Hillen (Eur. J. Biochem. 270) the alterations in rtTA-S2 that are responsible for the DNA binding of the modiﬁed transactivators is efﬁcient; reverse phenotype . None of the exchanges found in in transient transfections in HeLa cells, they speciﬁcally these new alleles were present in the original rtTA. The achieve induction factors between 2000 and 8000 and are, mutations leading to the reverse phenotype are located at thus, as active as wild-type tTA2. Moreover, they are also the interface between the DNA reading head and the highly speciﬁc, as they induce the converse operator less protein core or in the last turn of helix a6 that undergoes a than twofold . Modulation of the DNA-binding conformational change upon inducer binding. Structural speciﬁcity is not conﬁned to tTA. Alleles speciﬁc for the analysis of the DNA-bound form of TetR has led to the 4C-  and 6C-tet operators  have been constructed proposal that the mutations present in rtTA  restrict with rtTA and also regulate tc-dependent expression units the repressor to a noninducible conformation and lock the efﬁciently. This now leaves us with different tTA- and rtTA- DNA-binding domains in the position necessary for oper- operator combinations capable of controlling gene expres- ator binding . Taken together, the phenotype of rtTA can sion tightly over a wide range of inducer concentrations.
be improved and designed by using appropriate screens.
Mastering subunit recognition of TetR Tet transregulators vary in their sensitivity towards Comparison of the TetR primary structures reveals 38–90% tetracyclinic inducers identical amino acids overall, but only 18% in the four-helix The tTA and rtTA variants presently employed in eukary- bundle involved in dimerization. Detailed structural infor- otic gene regulation display differential sensitivity towards mation  of the dimerization interface  suggested that tc and its derivatives. While tTA can be induced by tc, TetR proteins from individual classes would not readily dox and atc , reverse transactivators respond only to form heterodimers. The modular architecture of TetR dox and atc  and tTSG is about twofold less sensitive to allows the combination of a class B DNA-binding domain dox than rtTA . The response range of tTA to dox (0.1– with the inducer-binding and/or dimerization domains of 10 ngÆmL)1) is clearly lower and, more importantly, non- Tet repressors from other classes . Fusion to the overlapping with that of rtTA to dox (100–3000 ngÆmL)1) reading head from TetR(B) increases activity of Tet , but slightly overlapping with that of the more sensitive repressors from several other classes  and ensures tight rtTA2s-M2 allele (2–200 ngÆmL)1) . The molecular binding to the tetO boxes from Tn10 . Class B TetR mechanisms responsible for these different sensitivities are does not form heterodimers with Tet repressors from classes presently unknown. The isolation of a tc-like antagonist for D , E [93,114,120], or G . The fusion points can be TetR  and the demonstration of its activity in chosen with some ﬂexibility; functional chimeras have been transgenic plants  make it seem likely that alternative obtained either by connecting the entire protein core from inducers for TetR can be identiﬁed by screening.
TetR(D) or TetR(E) to a TetR(B) DNA-reading head[114,120,121] or by replacing the four-helix bundle formedby the helices a8 and a10 from both subunits (see Fig. 2), The DNA binding specificity of Tet-transregulators with the respective region from TetR(G) . The resulting transrepressors or transactivators regulate gene expression Structure–function analyses of TetR–tetO interactions had efﬁciently and do not form heterodimers as demonstrated in shown that only few changes (shown in Fig. 4) in the DNA DNA-retardation assays , immunoprecipitation and binding helix–turn–helix motif of TetR sufﬁce to switch the FACS analysis  opening up the possibility to introduce recognition speciﬁcity from the 19-base pair wild-type tetO two or more TetR-based regulatory proteins into the same to variants containing symmetric exchanges of bases at cell without having to cope with the disadvantages of position 4 (tetO-4C ) and position 6 (tetO-6C ).
heterodimer formation [114,115].
The TetR mutants were converted into the transactivatorstTA24C or tTA26C and minimal promoters Ptet4 and Ptet6 Combinatorial Tet regulation solves special were constructed with the respective tetO variants .
problems and allows sophisticatedapplications The previous section has shown that DNA-binding speci-ﬁcity, subunit recognition and response to the inducer canbe altered in TetR. Fig. 5 gives an overview of the presentstate of the Tet modules that are available for use and thefollowing section presents a few principles of how themodular nature of the transregulators can be exploited toaddress speciﬁc experimental requirements and open up newapplications for conditional regulation.
Fig. 4. Operator speciﬁcity combinations for the Tet system. The pri-mary structure of the TetR(B) recognition helix a3 and the ﬂanking Expression can be switched between two alleles loops is given in standard one-letter abbreviations. The entire sequence of tetO2 is shown with the palindromic center marked by an asteriskand the base numbering shown above one operator half-side. The The expression of two genes or of two alleles of one gene can exchanges in TetR and tetO are highlighted in inverse print for each be controlled in a mutually exclusive manner by combining matching pair (wt, 4C, 6C).
different dimerization domains, different operator-binding
Gene regulation by tetracyclines (Eur. J. Biochem. 270) 3115 therapeutic goal has been reached or, if necessary, in caseof emergency.
One gene can be regulated stringently by converselyacting transrepressor and transactivator Detectable levels of transgene expression in animals or cellsin which the transactivator is not active can limit theusefulness of any conditional expression system for mode-ling complex biological processes or evaluating the effects ofa gene product. For the Tet system, this transgene leakagehas been attributed either to basal activity of the respectivetetO-based minimal promoter used (see above; ); or, insystems with rtTA, to residual binding of the reversetransactivator to tetO in the absence of dox [123,124]. Astringently controlled regulatory system can now be Fig. 5. The Tet toolbox. TetR modules and regulatory domains are accomplished by combining a trans-silencer with a reverse displayed with the possible combinations. The diﬀerent binding func- transactivator, since heterodimer formation and concomit- tions of TetR were coded in diﬀerent shades of gray and placed at theirapproximate position in the protein, but not drawn to scale. The TetR ant phenotype blurring will be prevented if the trans-silencer variants characterized were classiﬁed in the corresponding module.
is equipped with a dimerization domain from the TetR The regulatory domains that can be fused to TetR are coded in dif- classes E or G. Thus, both transregulators bind in a ferent shades of gray according to their viral, human, insect or fungal mutually exclusive manner. Gene expression is actively origin. Note that not every possible combination of modules need repressed in the absence of dox by the binding of tTSE/tTSG result in a transregulator with acceptable regulatory properties.
to the minimal promoter. Upon addition of dox, tTSE/tTSGdissociates from tetO, allowing the reverse transactivator tobind and activate transcription. This setup efﬁciently speciﬁcities and by exploiting the differential sensitivity of reduces background expression in yeast , in mammalian Tet transregulators towards tetracyclines . Interference cell lines [93,115,120] and in transgenic animals [125–127], between the two expression units is excluded by using a tTA while affecting the maximal expression level only slightly allele with an alternative class E or G dimerization domain  or not at all .
and by furnishing rtTA with a modiﬁed DNA-bindingdomain that contacts the tetO-4C operator in Ptet4 speci- Transgenes can be expressed in a graded ﬁcally. Expression of the wild-type allele, for example, is or in a binary manner placed under tTA control and represents the normal state ofthe cell. A knockout situation can be generated by adding Transcriptional control has generally been assumed to either tc or, alternatively, atc or dox at concentrations operate as a binary switch with on/off characteristics between 10 and 100 ngÆmL)1 which dissociates tTA from [129,130], but several examples displaying graded changes the promoter but does not lead to DNA binding by rtTA in gene expression have recently been published [131,132].
. Maintaining the intermediate concentration of dox The manner of gene expression might well be a key factor in needed to shut down expression of both alleles will be programs of cell differentiation or stimulus response.
feasible in cell culture applications. In transgenic animals, Different regulatory setups of the Tet system allow a however, the necessary ﬁne-tuning of a dox or atc concen- transgene to be expressed in one or the other manner [133– tration may prove impossible suggesting instead the use of tc 135], enabling not only an analysis of a gene's function, but to shut down tTA-dependent gene expression without also of its mode of expression. When tTA and rtTA are interfering with regulation by rtTA. To switch to the expressed constitutively in mammalian cells and also in expression of the mutant allele requires atc or dox concen- S. cerevisiae, they drive transgene expression in a dose- trations of 1 lgÆmL)1 or more.
dependent, graded manner [133,135]. However, when rtTA Such a dual control system can provide valuable was expressed in S. cerevisiae under conditions of positive insights into developmental and pathogenic processes.
feedback using an autoregulatory circuit, the cell population One can imagine shutting down expression of a tumor was clearly divided into regulator-expressing and nonex- suppressor while inducing expression of an oncogene to pressing cell pools . In mammalian cells, the combined study cancerogenesis. Switching off expression of the usage of tTSG and rtTA also led to bimodal expression of oncogene after tumor formation can establish whether the the GFP reporter (see Fig. 3 in ). Although not respective protein is a valid target for therapeutic inter- formally proven, we assume that a bimodal expression vention. One could also switch from a wild-type to a pattern will not be observed for all repressor/activator mutant allele at a deﬁned developmental state of the combinations, but only for those in which the sensitivity of organism and then return to wild-type expression at a later tTS for the inducer is lower than that of the rtTA allele used, stage. This type of regulatory circuit can also deliver an as is the case for tTSG (compare the dose–response curve of additional degree of freedom to gene therapeutic strat- tTSE and rtTA of Fig. 4 in  with the one for tTSG and egies ) one regulatory circuit may be used to control a rtTA of Fig. 2 of ). This will ensure that rtTA is therapeutic gene, while the other may be exploited to serve preloaded with inducer and ready to activate transcription as a suicide switch to terminate the treatment once the the moment the dox concentrations needed for binding to 3116 C. Berens and W. Hillen (Eur. J. Biochem. 270) tTSG are reached and tetO is subsequently released. In fragment in the brain demonstrated that its continuous principle, only two regulatory states are observed: either the supply was needed to maintain the characteristic neuro- tetO sites are fully occupied with tTSG and gene expression pathology and behavioral phenotype, raising the possibility is shut off, or they are saturated with the rtTA variant, that the disease may be reversible by targeting the causative resulting in full transcriptional activation. The consequence agent .
is a binary expression pattern of the target gene. While this Regulation by the Tet system has also had a signiﬁcant setup already works with rtTA, the effect should be even impact on behavioral studies. Expression of constitutively more pronounced with rtTA2s-M2, as its inducer response active mutant forms of the calcium/calmodulin dependent range overlaps completely with that of tTSG.
kinase II or calcineurin in the brain of adult mice resulted inaltered synaptic plasticity and impairments in spatial Highlighting the regulatory potential memory storage and retrieval, but these deﬁcits were fully and looking into the future reversed when transgene expression was suppressed[84,152]. Because expression of the transgene was limited The properties and the adaptability of Tet regulation as to the hippocampus, this structure was additionally proven presented in the previous sections allow its use in many to be the site responsible for the behavioral effects. In a different applications. We would like to demonstrate this different example, knockout mice lacking the serotonin 1A enormous variability by referring to a few key studies that, receptor show increased anxiety-like behavior which could in our opinion, highlight the potential of Tet regulation.
be rescued by conditional expression, but only if the Regulation by tetracyclines is sensitive and efﬁcient receptor was synthesized during the early postnatal period enough to control target gene expression in pathogenic in the hippocampus and cortex .
organisms even when they have been injected into a Nevertheless, improvement and additions to the Tet mammalian host. The role of individual genes in infection system, among them the regulatory components, are still and pathogenesis can, thus, be probed and their validity as possible and necessary. Promoter development has not targets for therapeutic intervention determined in an in vivo received the same degree of attention as the transregulators.
disease model . This has not only been demonstrated The number of tetO elements and their spacing , as well for trypanosomes [137,138], but also for common human as the linker sequence separating the operators  have pathogens like Staphylococcus aureus  and Candida not been optimized yet. It remains to be seen if an ideal glabrata . In the fungus, squalene synthase  and minimal promoter with no intrinsic leakiness supporting sterol 14a-demethylase  were, thus, shown not to be very high-level activation can be identiﬁed or designed.
ideal targets for antifungal development.
Fortunately, screens for regulators with improved prop- The successful expression of the diphtheria toxin A erties can now be performed in eukaryotic systems  subunit by tTA/Ptet-1 in transgenic mice has demonstrated and, as an example, the isolation of novel Tet regulators the stringency of regulation that can be reached with the Tet which recognize nonantibiotically active tetracyclines or system . Although mouse lines that carried the target even nontetracyclinic inducers, would be of great beneﬁt.
transgene were obtained at an approximately 10-fold lower They would not only facilitate gene therapy applications frequency than normal, those that were established regula- which, at the moment, can be impaired by the use of ted the transgene efﬁciently. Induction of toxin expression tetracyclines in anti-infective therapy or their misuse as led to cell death and development of cardiomyopathies.
growth promoting additives to animal food. If these novel Stringent control of transgene expression using rtTA has inducers are not only ecologically safe, but also easy and also been achieved in HeLa cells for the Shiga toxin B nonexpensive to manufacture, the inducer–regulator pair- subunit , for the proapoptotic gene PUMA in SAOS-2 ing could also be useful in insect population control using and H1299 cell lines  and, using rtTA2s-S2 in transgenic dominant, repressible, lethal genetic systems [156,157] and mice, for Cre-recombinase .
might even introduce regulation by the Tet system to crop The strength of a true conditional system ) the possibi- plants. They would add to the repertoire of transregulators lity to switch gene expression on and off at leisure and and ﬁnally, since multiple dimerization and DNA-binding repeatedly ) represents a powerful method with which to speciﬁcities are already present, allow fully independent explore the relationship between mutant protein expression expression control of more than one gene by the Tet and disease progression. This has become evident upon studying transgenic mouse models for cancer and neuro- A major experimental challenge will be to express a target logical disorders. Here, the use of tTA and rtTA to control gene within its physiological window, which might depend expression of an oncogene revealed for solid tumors on environmental stimuli and even change during develop- [146,147] and for leukemias [148,149] that the oncogene is ment, since over- or underexpression often results in altered not only necessary for tumor formation but also for tumor phenotypes  or pathologies. While tc-controlled expres- maintenance, suggesting pharmacological inactivation of sion can mimic the natural level , this must not always oncogenes as a possible therapeutic strategy for cancer. This be the case. A solution might be precise promoter targeting assumption has been substantiated by the unexpected by tetO elements, to minimally interfere with gene expres- observation that, after having gone through one cycle of sion. This will be difﬁcult and will require extensive MYC-gene expression and silencing, reactivation of the knowledge about the inﬂuence of chromatin structure on oncogene does not lead to tumor regrowth, but rather to gene expression and its sensitivity to perturbation, partic- apoptosis . Similar effects have also been found for ularly when regulatory regions are modiﬁed . But, if neurological disorders. In a conditional model of Hunting- successful, this approach will provide an additional degree ton's disease, mice expressing a mutated huntingtin of freedom to manipulate gene expression, as the existing Gene regulation by tetracyclines (Eur. J. Biochem. 270) 3117 transregulators can be used to activate or silence gene 6. Hillen, W. & Berens, C. (1994) Mechanisms underlying expres- expression, in addition to and independent of the promo- sion of Tn10 encoded tetracycline resistance. Annu. Rev. Micro- ter's natural expression pattern.
biol. 48, 345–369.
7. Takahashi, M., Altschmied, L. & Hillen, W. (1986) Kinetic and equilibrium characterization of the Tet repressor-tetracycline complex by ﬂuorescence measurements. Evidence for divalentmetal ion requirement and energy transfer. J. Mol. Biol. 187, The Tet system is the most widely used regulatory system for conditional gene expression at the moment. The 8. Orth, P., Schnappinger, D., Hillen, W., Saenger, W. & Hinrichs, increasing number of: (a) cell lines stably transfected with W. (2000) Structural basis of gene regulation by the tetracycline tTA and rtTA; (b) cell lines harboring tTA or rtTA that inducible Tet repressor-operator system. Nature Struct. Biol. 7, have been derived from transgenic mice; and (c) transgenic mice expressing either the transregulators via cell-type 9. Lederer, T., Kintrup, M., Takahashi, M., Sum, P.E., Ellestad, speciﬁc promoters or a target gene under Ptet-1 control will G.A. & Hillen, W. (1996) Tetracycline analogs aﬀecting binding greatly facilitate genetic studies by allowing combination of to Tn10-encoded Tet repressor trigger the same mechanism of the existing components instead of having to generate all cell induction. Biochemistry 35, 7439–7446.
and mouse lines, a costly and time-consuming process.
10. McMurry, L., Petrucci, R.E. Jr & Levy, S.B. (1980) Active eﬄux Ongoing improvement of the existing components as well as of tetracycline encoded by four genetically diﬀerent tetracycline the continuous addition of new components to extend its resistance determinants in Escherichia coli. Proc. Natl Acad. Sci.
applicability have turned the Tet system into a highly USA 77, 3974–3977.
11. Moyed, H.S., Nguyen, T.T. & Bertrand, K.P. (1983) Multicopy versatile and ﬂexible regulatory system that can be adapted Tn10 tet plasmids confer sensitivity to induction of tet gene to many different applications. Starting from an extensive expression. J. Bacteriol. 155, 549–556.
knowledge-base of TetR structure–activity relationships 12. Nguyen, T.N., Phan, Q.G., Duong, L.P., Bertrand, K.P. & and the strength of the genetic screening and selection Lenski, R.E. (1989) Eﬀects of carriage and expression of the Tn10 systems in both pro- and eukaryotes, the Tet system is tetracycline-resistance operon on the ﬁtness of Escherichia coli becoming more and more capable of modeling the sophis- K12. Mol. Biol. Evol. 6, 213–225.
ticated regulatory setups needed [48,51] to analyze complex 13. Berg, C.M., Liu, L., Wang, B. & Wang, M.D. (1988) Rapid and multifactor biological processes in development and identiﬁcation of bacterial genes that are lethal when cloned on disease, thereby not only improving our understanding of multicopy plasmids. J. Bacteriol. 170, 468–470.
living organisms, but also revealing novel and innovative 14. Eckert, B. & Beck, C.F. (1989) Overproduction of transposon approaches to treat maladies.
Tn10-encoded tetracycline resistance protein results in cell deathand loss of membrane potential. J. Bacteriol. 171, 3557–3559.
15. Bertrand, K.P., Postle, K., Wray, L.V. Jr & Reznikoﬀ, W.S.
(1984) Construction of a single-copy promoter vector and its usein analysis of regulation of the transposon Tn10 tetracycline This work was supported by the Bayerische Forschungsstiftung resistance determinant. J. Bacteriol. 158, 910–919.
through their FORGEN initiative, by the Deutsche Forschungsgeme- 16. Epe, B. & Woolley, P. (1984) The binding of 6-demethyl- inschaft through SFB473 and the Fonds der Chemischen Industrie chlortetracycline to 70S, 50S and 30S ribosomal particles: a Deutschlands. We would also like to thank Dr Anja Knott and Felix quantitative study by ﬂuorescence anisotropy. EMBO J. 3, Kuphal for critical reading of the manuscript.
17. Orth, P., Cordes, F., Schnappinger, D., Hillen, W., Saenger, W. & Hinrichs, W. (1998) Conformational changes of the Tet repressorinduced by tetracycline trapping. J. Mol. Biol. 279, 439–447.
1. Yamaguchi, A., Udagawa, T. & Sawai, T. (1990) Transport of 18. Hinrichs, W., Kisker, C., Du¨vel, M., Mu¨ller, A., Tovar, K., divalent cations with tetracycline as mediated by the transposon Hillen, W. & Saenger, W. (1994) Structure of the Tet repressor- Tn10-encoded tetracycline resistance protein. J. Biol. Chem. 265, tetracycline complex and regulation of antibiotic resistance.
Science 264, 418–420.
2. Yamaguchi, A., Iwasaki-Ohba, Y., Ono, N., Kaneko-Ohdera, 19. Kisker, C., Hinrichs, W., Tovar, K., Hillen, W. & Saenger, W.
M. & Sawai, T. (1991) Stoichiometry of metal-tetracycline/H+ (1995) The complex formed between Tet repressor and tetra- antiport mediated by transposon Tn10-encoded tetracycline cycline-Mg2+ reveals mechanism of antibiotic resistance. J. Mol.
resistance protein in Escherichia coli. FEBS Lett. 282, 415–418.
Biol. 247, 260–280.
3. Levy, S.B., McMurry, L.M., Barbosa, T.M., Burdett, V., 20. Orth, P., Saenger, W. & Hinrichs, W. (1999) Tetracycline- Courvalin, P., Hillen, W., Roberts, M.C., Rood, J.I. & Taylor, chelated Mg2+ ion initiates helix unwinding in Tet repressor D.E. (1999) Nomenclature for new tetracycline resistance induction. Biochemistry 38, 191–198.
determinants. Antimicrob. Agents Chemother. 43, 1523–1524.
21. Orth, P., Schnappinger, D., Sum, P.E., Ellestad, G.A., Hillen, W., 4. Tauch, A., Pu¨hler, A., Kalinowski, J. & Thierbach, G. (2000) Saenger, W. & Hinrichs, W. (1999) Crystal structure of the TetZ, a new tetracycline resistance determinant discovered in Tet repressor in complex with a novel tetracycline, 9-(N,N- Gram-positive bacteria, shows high homology to Gram-negative regulated eﬄux systems. Plasmid 44, 285–291.
Biol. 285, 455–461.
5. Tauch, A., Go¨tker, S., Pu¨hler, A., Kalinowski, J. & Thierbach, G.
22. Saenger, W., Orth, P., Kisker, C., Hillen, W. & Hinrichs, W.
(2002) The 27.8-kb R-plasmid pTET3 from Corynebacterium (2000) The tetracycline repressor-A paradigm for a biological glutamicum encodes the aminoglycoside adenyltransferase gene switch. Angew. Chem. Int. Ed. Engl. 39, 2042–2052.
cassette aadA9 and the regulated tetracycline eﬄux system Tet 33 23. Matthews, K.S., Falcon, C.M. & Swint-Kruse, L. (2000) ﬂanked by active copies of the widespread insertion sequence Relieving repression. Nature Struct. Biol. 7, 184–187.
IS6100. Plasmid 48, 117–129.
3118 C. Berens and W. Hillen (Eur. J. Biochem. 270) 24. Mu¨ller, G., Hecht, B., Helbl, V., Hinrichs, W., Saenger, W. & 44. Shockett, P.E. & Schatz, D.G. (1996) Diverse strategies for tet- Hillen, W. (1995) Characterization of non-inducible Tet repressor racycline-regulated inducible gene expression. Proc. Natl Acad.
mutants suggests conformational changes necessary for induc- Sci. USA 93, 5173–5176.
tion. Nature Struct. Biol. 2, 693–703.
45. Blau, H.M. & Rossi, F.M.V. (1999) Tet B or not tet B: advances 25. Hecht, B., Mu¨ller, G. & Hillen, W. (1993) Noninducible Tet in tetracycline-inducible gene expression. Proc. Natl Acad. Sci.
repressor mutations map from the operator binding motif to the USA 96, 797–799.
C terminus. J. Bacteriol. 175, 1206–1210.
46. Baron, U. & Bujard, H. (2000) Tet repressor-based system for 26. Tiebel, B., Radzwill, N., Aung-Hilbrich, L.M., Helbl, V., regulated gene expression in eukaryotic cells: principles and Steinhoﬀ, H.J. & Hillen, W. (1999) Domain motions accom- advances. Methods Enzymol. 327, 401–421.
panying Tet repressor induction deﬁned by changes of interspin 47. Fussenegger, M. (2001) The impact of mammalian gene regula- distances at selectively labeled sites. J. Mol. Biol. 290, 229–240.
tion concepts on functional genomic research, metabolic engine- 27. Tiebel, B., Garke, K. & Hillen, W. (2000) Observing conforma- ering, and advanced gene therapies. Biotechnol. Prog. 17, 1–51.
tional and activity changes of Tet repressor in vivo. Nature Struct.
48. Lewandoski, M. (2001) Conditional control of gene expression in Biol. 7, 479–481.
the mouse. Nature Rev. Genet. 2, 743–755.
28. Tiebel, B., Aung-Hilbrich, L.M., Schnappinger, D. & Hillen, W.
49. Yamamoto, A., Hen, R. & Dauer, W.T. (2001) The ons and oﬀs (1998) Conformational changes necessary for gene regulation by of inducible transgenic technology: a review. Neurobiol. Dis. 8, Tet repressor assayed by reversible disulﬁde bond formation.
EMBO J. 17, 5112–5119.
50. Gossen, M. & Bujard, H. (2002) Studying gene function in 29. Berens, C., Schnappinger, D. & Hillen, W. (1997) The role of the eukaryotes by conditional gene inactivation. Annu. Rev. Genet.
variable region in Tet repressor for inducibility by tetracycline.
J. Biol. Chem. 272, 6936–6942.
51. Jonkers, J. & Berns, A. (2002) Conditional mouse models of 30. Kintrup, M., Schubert, P., Kunz, M., Chabbert, M., Alberti, P., sporadic cancer. Nature Rev. Cancer 2, 251–265.
Bombarda, E., Schneider, S. & Hillen, W. (2000) Trp scanning 52. Zhu, Z., Zheng, T., Lee, C.G., Homer, R.J. & Elias, J.A. (2002) analysis of Tet repressor reveals conformational changes asso- ciated with operator and anhydrotetracycline binding. Eur. J.
advances and application in transgenic animal modeling. Semin.
Biochem. 267, 821–829.
Cell Dev. Biol. 13, 121–128.
31. Scholz, O., Kintrup, M., Reich, M. & Hillen, W. (2001) 53. Gatz, C. & Quail, P.H. (1988) Tn10-encoded tet repressor can Mechanism of Tet repressor induction by tetracyclines: length regulate an operator-containing plant promoter. Proc. Natl Acad.
compensates for sequence in the a8-a9 loop. J. Mol. Biol. 310, Sci. USA 85, 1394–1397.
54. Gatz, C., Kaiser, A. & Wendenburg, R. (1991) Regulation of a 32. Argast, M. & Beck, C.F. (1984) Tetracycline diﬀusion through modiﬁed CaMV 35S promoter by the Tn10-encoded Tet phospholipid bilayers and binding to phospholipids. Antimicrob.
repressor in transgenic tobacco. Mol. Gen. Genet. 227, 229–237.
Agents Chemother. 26, 263–265.
55. Yan, S., Myler, P.J. & Stuart, K. (2001) Tetracycline regulated 33. Argast, M. & Beck, C.F. (1985) Tetracycline uptake by suscep- gene expression in Leishmania donovani. Mol. Biochem. Parasitol.
tible Escherichia coli cells. Arch. Microbiol. 141, 260–265.
34. Katiyar, S.K. & Edlind, T.D. (1991) Enhanced antiparasitic 56. Wirtz, E. & Clayton, C. (1995) Inducible gene expression in activity of lipophilic tetracyclines: role of uptake. Antimicrob.
trypanosomes mediated by a prokaryotic repressor. Science 268, Agents Chemother. 35, 2198–2202.
35. Pezeshk, A., Pezeshk, V., Firlej, A., Wojas, J. & Subczynski, 57. Dingermann, T., Frank-Stoll, U., Werner, H., Wissmann, A., W.K. (1993) Transport of spin-labeled tetracycline across model Hillen, W., Jacquet, M. & Marschalek, R. (1992) RNA poly- and biological membranes. Life Sci. 52, 1071–1078.
merase III catalysed transcription can be regulated in Saccharo- 36. Sigler, A., Schubert, P., Hillen, W. & Niederweis, M. (2000) myces cerevisiae by the bacterial tetracycline repressor-operator Permeation of tetracyclines through membranes of liposomes and system. EMBO J. 11, 1487–1492.
Escherichia coli. Eur. J. Biochem. 267, 527–534.
58. Dingermann, T., Werner, H., Schu¨tz, A., Zu¨ndorf, I., Nerke, K., 37. Sumita, Y. & Shishido, K. (1985) Regulation of tetracycline Knecht, D. & Marschalek, R. (1992) Establishment of a system accumulation in Bacillus subtilis bearing B. subtilis plasmid for conditional gene expression using an inducible tRNA sup- pNS1981. FEMS Microbiol. Lett. 30, 403–406.
pressor gene. Mol. Cell. Biol. 12, 4038–4045.
38. Hutchings, B.L. (1969) Tetracycline transport in Staphylococcus 59. Ulmasov, B., Capone, J. & Folk, W. (1997) Regulated expression aureus H. Biochim. Biophys. Acta 174, 734–748.
of plant tRNA genes by the prokaryotic tet and lac repressors.
39. Yamaguchi, A., Ohmori, H., Kaneko-Ohdera, M., Nomura, Plant Mol. Biol. 35, 417–424.
T. & Sawai, T. (1991) DpH-dependent accumulation of 60. Ohkawa, J. & Taira, K. (2000) Control of the functional activity tetracycline in Escherichia coli. Antimicrob. Agents Chemother. 35, of an antisense RNA by a tetracycline-responsive derivative of the human U6 snRNA promoter. Hum. Gene Ther. 11, 577–585.
40. Nikaido, H. & Thanassi, D.G. (1993) Penetration of lipophilic 61. Weinberg, R.A. & Penman, S. (1968) Small molecular weight agents with multiple protonation sites into bacterial cells: tetra- monodisperse nuclear RNA. J. Mol. Biol. 38, 289–304.
cyclines and ﬂuoroquinolones as examples. Antimicrob. Agents 62. Hutva´gner, G. & Zamore, P.D. (2002) A microRNA in a mul- Chemother. 37, 1393–1399.
tiple-turnover RNAi enzyme complex. Science 297, 2056–2060.
41. Thanassi, D.G., Suh, G.S. & Nikaido, H. (1995) Role of outer 63. Yu, J.Y., DeRuiter, S.L. & Turner, D.L. (2002) RNA interference membrane barrier in eﬄux-mediated tetracycline resistance of by expression of short-interfering RNAs and hairpin RNAs in Escherichia coli. J. Bacteriol. 177, 998–1007.
mammalian cells. Proc. Natl Acad. Sci. USA 99, 6047–6052.
42. Gossen, M., Bonin, A.L. & Bujard, H. (1993) Control of gene 64. McManus, M.T. & Sharp, P.A. (2002) Gene silencing in activity in higher eukaryotic cells by prokaryotic regulatory ele- mammals by small interfering RNAs. Nature Rev. Genet. 3, ments. Trends Biochem. Sci. 18, 471–475.
43. Saez, E., No, D., West, A. & Evans, R.M. (1997) Inducible gene 65. Frohberg, C., Heins, L. & Gatz, C. (1991) Characterization of expression in mammalian cells and transgenic mice. Curr. Opin.
the interaction of plant transcription factors using a bacterial Biotechnol. 8, 608–616.
repressor protein. Proc. Natl Acad. Sci. USA 88, 10470–10474.
Gene regulation by tetracyclines (Eur. J. Biochem. 270) 3119 66. Heins, L., Frohberg, C. & Gatz, C. (1992) The Tn10-encoded Tet 85. Saam, J.R. & Gordon, J.I. (1999) Inducible gene knockouts in the repressor blocks early but not late steps of assembly of the RNA small intestinal and colonic epithelium. J. Biol. Chem. 274, polymerase II initiation complex in vivo. Mol. Gen. Genet. 232, 86. Stebbins, M.J. & Yin, J.C. (2001) Adaptable doxycycline- 67. Yao, F., Svensjo¨, T., Winkler, T., Lu, M., Eriksson, C. & regulated gene expression systems for Drosophila. Gene 270, Eriksson, E. (1998) Tetracycline repressor, tetR, rather than the tetR-mammalian cell transcription factor fusion derivatives, 87. Wells, K.D., Foster, J.A., Moore, K., Pursel, V.G. & Wall, R.J.
regulates inducible gene expression in mammalian cells. Hum.
(1999) Codon optimization, genetic insulation, and an rtTA Gene Ther. 9, 1939–1950.
reporter improve performance of the tetracycline switch. Trans- 68. Faryar, K. & Gatz, C. (1992) Construction of a tetracycline- genic Res. 8, 371–381.
inducible promoter in Schizosaccharomyces pombe. Curr. Genet.
88. Urlinger, S., Baron, U., Thellmann, M., Hasan, M.T., Bujard, H.
& Hillen, W. (2000) Exploring the sequence space for tetra- 69. Forsburg, S.L. (1993) Comparison of Schizosaccharomyces cycline-dependent transcriptional activators: novel mutations pombe expression systems. Nucleic Acids Res. 21, 2955–2956.
yield expanded range and sensitivity. Proc. Natl Acad. Sci. USA 70. Hamann, L., Buß, H. & Tannich, E. (1997) Tetracycline-con- 97, 7963–7968.
trolled gene expression in Entamoeba histolytica. Mol. Biochem.
89. Stebbins, M.J., Urlinger, S., Byrne, G., Bello, B., Hillen, W. & Parasitol. 84, 83–91.
Yin, J.C. (2001) Tetracycline-inducible systems for Drosophila.
71. Ramakrishnan, G., Vines, R.R., Mann, B.J. & Petri,W.A. Jr Proc. Natl Acad. Sci. USA 98, 10775–10780.
(1997) A tetracycline-inducible gene expression system in 90. Anastassiadis, K., Kim, J., Daigle, N., Sprengel, R., Scho¨ler, Entamoeba histolytica. Mol. Biochem. Parasitol. 84, 93–100.
H.R. & Stewart, A.F. (2002) A predictable ligand regulated 72. Meissner, M., Brecht, S., Bujard, H. & Soldati, D. (2001) expression strategy for stably integrated transgenes in mamma- Modulation of myosin A expression by a newly established lian cells in culture. Gene 298, 159–172.
tetracycline repressor-based inducible system in Toxoplasma 91. Ackland-Berglund, C.E. & Leib, D.A. (1995) Eﬃcacy of tetra- gondii. Nucleic Acids Res. 29, e115.
cycline-controlled gene expression is inﬂuenced by cell type.
73. Sun, C.-H. & Tai, J.-H. (2000) Development of a tetra- Biotechniques 18, 196–200.
cycline controlled gene expression system in the parasitic 92. Howe, J.R., Skryabin, B.V., Belcher, S.M., Zerillo, C.A. & protozoan Giardia lamblia. Mol. Biochem. Parasitol. 105, Schmauss, C. (1995) The responsiveness of a tetracycline-sensitive expression system diﬀers in diﬀerent cell lines. J. Biol. Chem. 270, 74. Kim, H.-J., Gatz, C., Hillen, W. & Jones, T.R. (1995) Tetra- cycline repressor-regulated gene repression in recombinant 93. Freundlieb, S., Schirra-Mu¨ller, C. & Bujard, H. (1999) A tetra- human cytomegalovirus. J. Virol. 69, 2565–2573.
cycline controlled activation/repression system with increased 75. Gossen, M. & Bujard, H. (1992) Tight control of gene expression potential for gene transfer into mammalian cells. J. Gene Med. 1, in mammalian cells by tetracycline-responsive promoters. Proc.
Natl Acad. Sci. USA 89, 5547–5551.
94. Freundlieb, S., Baron, U., Bonin, A.L., Gossen, M. & Bujard, H.
76. Baron, U., Gossen, M. & Bujard, H. (1997) Tetracycline- (1997) Use of tetracycline-controlled gene expression systems to controlled transcription in eukaryotes: novel transactivators study mammalian cell cycle. Methods Enzymol. 283, 159–173.
with graded transactivation potential. Nucleic Acids Res. 25, 95. Hoﬀmann, A., Villalba, M., Journot, L. & Spengler, D. (1997) A novel tetracycline-dependent expression vector with low basal 77. Nagahashi, S., Nakayama, H., Hamada, K., Yang, H., Arisawa, expression and potent regulatory properties in various mamma- M. & Kitada, K. (1997) Regulation by tetracycline of gene lian cell lines. Nucleic Acids Res. 25, 1078–1079.
expression in Saccharomyces cerevisiae. Mol. Gen. Genet. 255, 96. Leuchtenberger, S., Perz, A., Gatz, C. & Bartsch, J.W. (2001) Conditional cell ablation by stringent tetracycline-dependent 78. Urlinger, S., Helbl, V., Guthmann, J., Pook, E., Grimm, S. & regulation of barnase in mammalian cells. Nucleic Acids Res. 29, Hillen, W. (2000) The p65 domain from NF-jB is an eﬃcient human activator in the tetracycline-regulatable gene expression 97. Margolin, J.F., Friedman, J.R., Meyer, W.K., Vissing, H., system. Gene 247, 103–110.
Thiesen, H.J., Rauscher, F.J. 3rd. (1994) Kru¨ppel-associated 79. Akagi, K., Kanai, M., Saya, H., Kozu, T. & Berns, A. (2001) A boxes are potent transcriptional repression domains. Proc. Natl novel tetracycline-dependent transactivator with E2F4 tran- Acad. Sci. USA 91, 4509–4513.
scriptional activation domain. Nucleic Acids Res. 29, e23.
98. Pikaart, M.J., Recillas-Targa, F. & Felsenfeld, G. (1998) Loss of 80. Go, W.Y. & Ho, S.N. (2002) Optimization and direct comparison transcriptional activity of a transgene is accompanied by DNA of the dimerizer and reverse tet transcriptional control systems.
methylation and histone deacetylation and is prevented by J. Gene Med. 4, 258–270.
insulators. Genes Dev. 12, 2852–2862.
81. Deuschle, U., Meyer, W.K. & Thiesen, H.J. (1995) Tetracycline- 99. Pannell, D. & Ellis, J. (2001) Silencing of gene expression: reversible silencing of eukaryotic promoters. Mol. Cell. Biol. 15, implications for design of retrovirus vectors. Rev. Med. Virol. 11, 82. Bellı´, G., Garı´, E., Piedraﬁta, L., Aldea, M. & Herrero, E. (1998) 100. Gopalkrishnan, R.V., Christiansen, K.A., Goldstein, N.I., An activator/repressor dual system allows tight tetracycline- DePinho, R.A. & Fisher, P.B. (1999) Use of the human EF-1a regulated gene expression in budding yeast. Nucleic Acids Res. 26, promoter for expression can signiﬁcantly increase success in establishing stable cell lines with consistent expression: a study 83. Ryu, J.R., Olson, L.K. & Arnosti, D.N. (2001) Cell-type speci- using the tetracycline-inducible system in human cancer cells.
ﬁcity of short-range transcriptional repressors. Proc. Natl Acad.
Nucleic Acids Res. 27, 4775–4782.
Sci. USA 98, 12960–12965.
101. Izumi, M. & Gilbert, D.M. (1999) Homogeneous tetracycline- 84. Mayford, M., Bach, M.E., Huang, Y.Y., Wang, L., Hawkins, regulatable gene expression in mammalian ﬁbroblasts. J. Cell.
R.D. & Kandel, E.R. (1996) Control of memory formation Biochem. 76, 280–289.
through regulated expression of a CaMKII transgene. Science 102. Callus, B.A. & Mathey-Prevot, B. (1999) Rapid selection 274, 1678–1683.
of tetracycline-controlled inducible cell lines using a green 3120 C. Berens and W. Hillen (Eur. J. Biochem. 270) ﬂuorescent-transactivator fusion protein. Biochem. Biophys. Res.
reduced basal activity in mammalian cells. Nucleic Acids Res. 27, Commun. 257, 874–878.
103. Johansen, J., Rosenblad, C., Andsberg, K., Møller, A., Lund- 121. Schnappinger, D., Schubert, P., Pﬂeiderer, K. & Hillen, W. (1998) berg, C., Bjo¨rlund, A. & Johansen, T.E. (2002) Evaluation of Determinants of protein-protein recognition by four helix bun- Tet-on system to avoid transgene down-regulation in ex vivo gene dles: changing the dimerization speciﬁcity of Tet repressor.
transfer to the CNS. Gene Ther. 9, 1291–1301.
EMBO J. 17, 535–543.
104. Wissmann, A., Wray, L.V. Jr, Somaggio, U., Baumeister, R., 122. Altschmied, L., Baumeister, R., Pﬂeiderer, K. & Hillen, W. (1988) Geissendo¨rfer, M. & Hillen, W. (1991) Selection for Tn10 Tet A threonine to alanine exchange at position 40 of Tet repressor repressor binding to tet operator in Escherichia coli: isolation of alters the recognition of the sixth base pair of tet operator from temperature-sensitive mutants and combinatorial mutagenesis in GC to AT. EMBO J. 7, 4011–4017.
the DNA binding motif. Genetics 128, 225–232.
123. Lindeberg, J. & Ebendal, T. (1999) Use of an internal ribosome 105. Biburger, M., Berens, C., Lederer, T., Krec, T. & Hillen, W.
entry site for bicistronic expression of Cre recombinase or rtTA (1998) Intragenic suppressors of induction-deﬁcient TetR transactivator. Nucleic Acids Res. 27, 1552–1554.
mutants: localization and potential mechanism of action.
124. Rossant, J. & McMahon, A. (1999) Cre-ating mouse mutants-a J. Bacteriol. 180, 737–741.
meeting review on conditional mouse genetics. Genes Dev. 13, 106. Ashley, R.L., Corey, L., Dalessio, J., Wilson, P., Remington, M., Barnum, G. & Trethewey, P. (1994) Protein-speciﬁc cervical 125. Zhu, Z., Ma, B., Homer, R.J., Zheng, T. & Elias, J.A. (2001) Use antibody responses to primary genital herpes simplex virus type 2 of the tetracycline-controlled transcriptional silencer (tTS) to infections. J. Infect. Dis. 170, 20–26.
eliminate transgene leak in inducible overexpression transgenic 107. Doherty, D.G., Penzotti, J.E., Koelle, D.M., Kwok, W.W., mice. J. Biol. Chem. 276, 25222–25229.
Lybrand, T.P., Masewicz, S. & Nepom, G.T. (1998) Structural 126. Perez, N., Plence, P., Millet, V., Greuet, D., Minot, C., Noel, D., basis of speciﬁcity and degeneracy of T cell recognition: plur- Danos, O., Jorgensen, C. & Apparailly, F. (2002) Tetracycline iallelic restriction of T cell responses to a peptide antigen involves transcriptional silencer tightly controls transgene expression after both speciﬁc and promiscuous interactions between the T cell in vivo intramuscular electrotransfer: Application to Interleukin receptor, peptide, and HLA-DR. J. Immunol. 161, 3527–3535.
10 therapy in experimental arthritis. Hum. Gene Ther. 13, 2161– 108. Mikloska, Z. & Cunningham, A.L. (1998) Herpes simplex virus type 1 glycoproteins gB, gC and gD are major targets for CD4 127. Salucci, V., Scarito, A., Aurisicchio, L., Lamartina, S., Nicolaus, T-lymphocyte cytotoxicity in HLA-DR expressing human G., Giampaoli, S., Gonzalez-Paz, O., Toniatti, C., Bujard, H., epidermal keratinocytes. J. Gen. Virol. 79, 353–361.
Hillen, W., Ciliberto, G. & Palombo, F. (2002) Tight control of 109. Bohl, D., Naﬀakh, N. & Heard, J.M. (1997) Long-term control gene expression by a helper-dependent adenovirus vector carry- of erythropoietin secretion by doxycycline in mice transplanted ing the rtTA2s-M2 tetracycline transactivator and repressor sys- with engineered primary myoblasts. Nature Med. 3, 299–305.
tem. Gene Ther. 9, 1415–1421.
110. Gill, G. & Ptashne, M. (1988) Negative eﬀect of the transcrip- 128. Knott, A., Garke, K., Urlinger, S., Guthmann, J., Mu¨ller, Y., tional activator GAL4. Nature 334, 721–724.
Thellmann, M. & Hillen, W. (2002) Tetracycline-dependent gene 111. Kistner, A., Gossen, M., Zimmermann, F., Jerecic, J., Ullmer, C., regulation: combinations of transregulators yield a variety of Lu¨bbert, H. & Bujard, H. (1996) Doxycycline-mediated quanti- expression windows. Biotechniques 32, 796–807.
tative and tissue-speciﬁc control of gene expression in transgenic 129. Walters, M.C., Fiering, S., Eidemiller, J., Magis, W., Groudine, mice. Proc. Natl Acad. Sci. USA 93, 10933–10938.
M. & Martin, D.I. (1995) Enhancers increase the probability but 112. Hasan, M.T., Scho¨nig, K., Berger, S., Graewe, W. & Bujard, H.
not the level of gene expression. Proc. Natl Acad. Sci. USA 92, (2001) Long-term, noninvasive imaging of regulated gene expression in living mice. Genesis 29, 116–122.
130. Hume, D.A. (2000) Probability in transcriptional regulation and 113. Gossen, M., Freundlieb, S., Bender, G., Mu¨ller, G., Hillen, W. & its implications for leukocyte diﬀerentiation and inducible gene Bujard, H. (1995) Transcriptional activation by tetracyclines in expression. Blood 96, 2323–2328.
mammalian cells. Science 268, 1766–1769.
131. Niwa, H., Miyazaki, J. & Smith, A.G. (2000) Quantitative 114. Baron, U., Schnappinger, D., Helbl, V., Gossen, M., Hillen, W.
expression of Oct-3/4 deﬁnes diﬀerentiation, dediﬀerentiation or & Bujard, H. (1999) Generation of conditional mutants in higher self-renewal of ES cells. Nat. Genet. 24, 372–376.
eukaryotes by switching between the expression of two genes.
132. Biggar, S.R. & Crabtree, G.R. (2001) Cell signaling can direct Proc. Natl Acad. Sci. USA 96, 1013–1018.
either binary or graded transcriptional responses. EMBO J. 20, 115. Rossi, F.M.V., Guicherit, O.M., Spicher, A., Kringstein, A.M., Fatyol, K., Blakely, B.T. & Blau, H.M. (1998) Tetracycline- 133. Kringstein, A.M., Rossi, F.M., Hofmann, A. & Blau, H.M.
regulatable factors with distinct dimerization domains allow (1998) Graded transcriptional response to diﬀerent concentra- reversible growth inhibition by p16. Nature Genet. 20, 389–393.
tions of a single transactivator. Proc. Natl Acad. Sci. USA 95, 116. Chrast-Balz, J. & Hooft van Huijsduijnen, R. (1996) Bi-direc- tional gene switching with the tetracycline repressor and a novel 134. Rossi, F.M.V., Kringstein, A.M., Spicher, A., Guicherit, O.M. & tetracycline antagonist. Nucleic Acids Res. 24, 2900–2904.
Blau, H.M. (2000) Transcriptional control: rheostat converted to 117. Love, J., Allen, G.C., Gatz, C. & Thompson, W.F. (2002) Dif- on/oﬀ switch. Mol. Cell 6, 723–728.
ferential Top10 promoter regulation by six tetracycline analogues 135. Becskei, A., Se´raphin, B. & Serrano, L. (2001) Positive in plant cells. J. Exp. Bot. 53, 1871–1877.
feedback in eukaryotic gene networks: cell diﬀerentiation 118. Helbl, V. & Hillen, W. (1998) Stepwise selection of TetR variants by graded to binary response conversion. EMBO J. 20, 2528– recognizing tet operator 4C with high aﬃnity and speciﬁcity.
J. Mol. Biol. 276, 313–318.
136. Nakayama, H., Izuta, M., Nakayama, N., Arisawa, M. & Aoki, 119. Helbl, V., Tiebel, B. & Hillen, W. (1998) Stepwise selection of Y. (2000) Depletion of the squalene synthase (ERG9) gene does TetR variants recognizing tet operator 6C with high aﬃnity and not impair growth of Candida glabrata in mice. Antimicrob.
speciﬁcity. J. Mol. Biol. 276, 319–324.
Agents Chemother. 44, 2411–2418.
120. Forster, K., Helbl, V., Lederer, T., Urlinger, S., Wittenburg, N. & 137. Krieger, S., Schwarz, W., Ariyanayagam, M.R., Fairlamb, A.H., Hillen, W. (1999) Tetracycline-inducible expression systems with Krauth-Siegel, R.L. & Clayton, C. (2000) Trypanosomes lacking Gene regulation by tetracyclines (Eur. J. Biochem. 270) 3121 trypanothione reductase are avirulent and show increased sensi- Chodosh, L.A. (2002) Conditional activation of Neu in the tivity to oxidative stress. Mol. Microbiol. 35, 542–552.
mammary epithelium of transgenic mice results in reversible 138. van Deursen, F.J., Shahi, S.K., Turner, C.M.R., Hartmann, C., pulmonary metastasis. Cancer Cell 2, 451–461.
Guerra-Giraldez, C., Matthews, K.R. & Clayton, C.E. (2001) 148. Felsher, D.W. & Bishop, J.M. (1999) Reversible tumorigenesis by Characterisation of the growth and diﬀerentiation in vivo and MYC in hematopoietic lineages. Mol. Cell 4, 199–207.
in vitro-of bloodstream-form Trypanosoma brucei strain TREU 149. Huettner, C.S., Zhang, P., Van Etten, R.A. & Tenen, D.G. (2000) 927. Mol. Biochem. Parasitol. 112, 163–171.
Reversibility of acute B-cell leukaemia induced by BCR-ABL1.
139. Ji, Y., Zhang, B., Van Horn, S.F., Warren, P., Woodnutt, G., Nature Genet. 24, 57–60.
Burnham, M.K. & Rosenberg, M. (2001) Identiﬁcation of critical 150. Jain, M., Arvanitis, C., Chu, K., Dewey, W., Leonhardt, E., staphylococcal genes using conditional phenotypes generated by Trinh, M., Sundberg, C.D., Bishop, J.M. & Felsher, D.W. (2002) antisense RNA. Science 293, 2266–2269.
Sustained loss of a neoplastic phenotype by brief inactivation of 140. Nakayama, H., Izuta, M., Nagahashi, S., Sihta, E.Y., Sato, Y., MYC. Science 297, 102–104.
Yamazaki, T., Arisawa, M. & Kitada, K. (1998) A controllable 151. Yamamoto, A., Lucas, J.J. & Hen, R. (2000) Reversal of neu- gene-expression system for the pathogenic fungus Candida glab- ropathology and motor dysfunction in a conditional model of rata. Microbiology 144, 2407–2415.
Huntington's disease. Cell 101, 57–66.
141. Nakayama, H., Nakayama, N., Arisawa, M. & Aoki, Y. (2001) 152. Mansuy, I.M., Mayford, M., Jacob, B., Kandel, E.R. & Bach, In vitro and in vivo eﬀects of 14a-demethylase (ERG11) depletion M.E. (1998) Restricted and regulated overexpression reveals in Candida glabrata. Antimicrob. Agents Chemother. 45, 3037– calcineurin as a key component in the transition from short-term to long-term memory. Cell 92, 39–49.
142. Lee, P., Morley, G., Huang, Q., Fischer, A., Seiler, S., Horner, 153. Gross, C., Zhuang, X., Stark, K., Ramboz, S., Oosting, R., J.W., Factor, S., Vaidya, D., Jalife, J. & Fishman, G.I. (1998) Kirby, L., Santarelli, L., Beck, S. & Hen, R. (2002) Serotonin1A Conditional lineage ablation to model human diseases. Proc. Natl receptor acts during development to establish normal anxiety-like Acad. Sci. USA 95, 11371–11376.
behaviour in the adult. Nature 416, 396–400.
143. Nakagawa, I., Nakata, M., Kawabata, S. & Hamada, S. (1999) 154. Marzio, G., Verhoef, K., Vink, M. & Berkhout, B. (2001) In vitro Regulated expression of the Shiga toxin B gene induces apoptosis evolution of a highly replicating, doxycycline-dependent HIV for in mammalian ﬁbroblastic cells. Mol. Microbiol. 33, 1190–1199.
applications in vaccine studies. Proc. Natl Acad. Sci. USA 98, 144. Nakano, K. & Vousden, K.H. (2001) PUMA, a novel proa- poptotic gene, is induced by p53. Mol. Cell 7, 683–694.
155. Rang, A. & Will, H. (2000) The tetracycline-responsive promoter 145. Scho¨nig, K., Schwenk, F., Rajewsky, K. & Bujard, H. (2002) contains functional interferon-inducible response elements.
Stringent doxycycline dependent control of CRE recombinase Nucleic Acids Res. 28, 1120–1125.
in vivo. Nucleic Acids Res. 30, e134.
156. Thomas, D.D., Donnelly, C.A., Wood, R.J. & Alphey, L.S.
146. Fisher, G.H., Wellen, S.L., Klimstra, D., Lenczowski, J.M., (2000) Insect population control using a dominant, repressible, Tichelaar, J.W., Lizak, M.J., Whitsett, J.A., Koretsky, A. & lethal genetic system. Science 287, 2474–2476.
Varmus, H.E. (2001) Induction and apoptotic regression of lung 157. Horn, C. & Wimmer, E.A. (2003) A transgene-based, embryo- adenocarcinomas by regulation of a K-Ras transgene in the speciﬁc lethality system for insect pest management. Nat. Bio- presence and absence of tumor suppressor genes. Genes Dev. 15, technol. 21, 64–70.
158. Lomvardas, S. & Thanos, D. (2002) Modifying gene expression 147. Moody, S.E., Sarkisian, C.J., Hahn, K.T., Gunther, E.J., Pickup, programs by altering core promoter chromatin architecture. Cell S., Dugan, K.D., Innocent, N., Cardiﬀ, R.D., Schnall, M.D. & 110, 261–271.
Original Investigation ASSOCIATION OF VA SURGEONS Effect of a Preoperative Decontamination Protocol onSurgical Site Infections in Patients Undergoing ElectiveOrthopedic Surgery With Hardware Implantation Serge P. Bebko, MD; David M. Green, MD; Samir S. Awad, MD, MPH IMPORTANCE Surgical site infections (SSIs), commonly caused by methicillin-resistantStaphylococcus aureus (MRSA), are associated with significant morbidity and mortality,specifically when hardware is implanted in the patient. Previously, we have demonstratedthat a preoperative decontamination protocol using chlorhexidine gluconate washcloths andintranasal antiseptic ointment is effective in eradicating MRSA in the nose and on the skin ofpatients.